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Invitrogen
MBD2 Recombinant Rabbit Monoclonal Antibody (24GB4570)
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FIGURE: 1 / 5
MBD2 Antibody (MA5-57474) in ICC/IF
Immunocytochemistry analysis of MBD2 in HepG2 cells. Sample was incubated with MBD2 monoclonal (Product # MA5-57474) antibody at a dilution of 1:1,000, followed by DAPI and Alexa Fluor 647. Protein abundance based on laser intensity and smart gain: Medium. Scale bar, 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57474
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB4570
Immunogen
A synthesized peptide derived from human MBD2 (aa 216-234)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.7 mg/mL
Amount
70 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: demethylase; DKFZp586O0821; methyl-CpG-binding protein MBD2; testis-specific methyl-CpG binding protein 2; unnamed protein product
Gene Aliases: DMTase; MBD2a; NY-CO-41
UniProt ID: (Human) Q9UBB5, (Mouse) Q9Z2E1
Entrez Gene ID: (Human) 8932, (Rat) 680172, (Mouse) 17191
negative regulation of transcription from RNA polymerase II promoter
ventricular cardiac muscle tissue development
chromatin remodeling
methylation-dependent chromatin silencing
regulation of transcription from RNA polymerase II promoter
response to mechanical stimulus
response to nutrient levels
response to estradiol
regulation of gene expression, epigenetic
maternal behavior
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
embryonic organ development
response to bisphenol A
cellular response to serotonin
positive regulation of Wnt signaling pathway
regulation of cell proliferation
protein-containing complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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