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Invitrogen
PRMT5 Recombinant Rabbit Monoclonal Antibody (24GB845)
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FIGURE: 1 / 4
PRMT5 Antibody (MA5-57628) in ICC/IF
Immunocytochemical staining of PRMT5 in Hela cells using PRMT5 Recombinant Monoclonal Antibody (Product # MA5-57628) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; Protein arginine methyltransferase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57628
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB845
Immunogen
A synthesized peptide derived from human PRMT5 (aa 40-92)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; homolog of fission yeast Skb1; Jak-binding protein 1; JBP1; JBP1; homodimer; shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; unnamed protein product
Gene Aliases: HRMT1L5; HSL7; IBP72; JBP1; SKB1; SKB1Hs
UniProt ID: (Human) O14744, (Mouse) Q8CIG8
Entrez Gene ID: (Human) 10419, (Mouse) 27374, (Rat) 364382
p53 binding
transcription corepressor activity
protein binding
methyltransferase activity
methyl-CpG binding
protein-arginine N-methyltransferase activity
protein-arginine omega-N symmetric methyltransferase activity
histone methyltransferase activity
identical protein binding
ribonucleoprotein complex binding
histone methyltransferase activity (H4-R3 specific)
macromolecular complex binding
protein heterodimerization activity
E-box binding
histone H3 methyltransferase activity
chromatin binding
histone methyltransferase activity (H2A-R3 specific)
negative regulation of transcription from RNA polymerase II promoter
spliceosomal snRNP assembly
chromatin remodeling
DNA-templated transcription, termination
regulation of transcription, DNA-templated
protein methylation
gene expression
regulation of gene expression
peptidyl-arginine methylation
circadian regulation of gene expression
peptidyl-arginine N-methylation
endothelial cell activation
hypermethylation of CpG island
negative regulation of cell differentiation
positive regulation of mRNA splicing, via spliceosome
positive regulation of oligodendrocyte differentiation
regulation of ERK1 and ERK2 cascade
cellular response to growth factor stimulus
Golgi ribbon formation
negative regulation of cGAS/STING signaling pathway
positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway
regulation of mitotic nuclear division
negative regulation of transcription, DNA-templated
liver regeneration
regulation of signal transduction by p53 class mediator
positive regulation of rRNA processing
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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