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Invitrogen

Phospho-IRF3 (Ser396) Recombinant Rabbit Monoclonal Antibody (PSH06-75)

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Phospho-IRF3 (Ser396) Recombinant Rabbit Monoclonal Antibody (PSH06-75)

Product Details

MA5-57852

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH06-75

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser396 of human IRF3.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Dry ice (international)

RRID

AB_3680876

Product Specific Information

Positive Control: HT-29 transfected with 2.5µg/mL Poly (I:C) for 6 hours cell lysate, J774A.1 transfected with 2.5µg/mL Poly (I:C) for 6 hours cell lysate, human testis tissue, HT-29 cells transfected withPoly (I:C).

Tissue Specificity: Low tissue specificity.

Subcellular Location: Cytoplasm, Nucleus, Mitochondrion.

Sequence Similarities: 83% Mouse/Rat.

Predicted band size: 47 kDa.

Target Information

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: IRF-3a; IRF-3b; IRF-3c; IRF-3d; IRF-3e; IRF-3f; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; mIRF3a; phospho IRF3; unnamed protein product

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Gene Aliases: C920001K05Rik; IIAE7; IRF-3

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UniProt ID: (Human) Q14653, (Mouse) P70671

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Entrez Gene ID: (Human) 3661, (Mouse) 54131

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding identical protein binding protein homodimerization activity sequence-specific DNA binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter positive regulation of cytokine production immune system process cytoplasmic pattern recognition receptor signaling pathway regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process cellular response to DNA damage stimulus cell surface receptor signaling pathway mRNA transcription programmed cell death positive regulation of type I interferon production positive regulation of interferon-alpha production positive regulation of interferon-beta production toll-like receptor 4 signaling pathway TRIF-dependent toll-like receptor signaling pathway MDA-5 signaling pathway regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of innate immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter defense response to virus type I interferon signaling pathway cellular response to exogenous dsRNA macrophage apoptotic process cellular response to virus antiviral innate immune response cGAS/STING signaling pathway positive regulation of cytokine production involved in inflammatory response response to bacterium regulation of gene expression lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide response to exogenous dsRNA regulation of inflammatory response positive regulation of type I interferon-mediated signaling pathway cellular response to lipopolysaccharide programmed necrotic cell death
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