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Invitrogen
ADSL Recombinant Rabbit Monoclonal Antibody (8J9E10)
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FIGURE: 1 / 1
ADSL Antibody (MA5-57910) in WB
Western blot analysis of ADSL in Hep G2 and HeLa (25 µg/lane). Samples were incubated overnight at 4°C with ADSL Recombinant Monoclonal Antibody (Product # MA5-57910) using a dilution of 1:3, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 60 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57910
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8J9E10
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.66 mg/mL
Amount
166 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
ADSL (adenylosuccinate lyase), also known as AMPS, ASL or ASASE, is a 484 amino acid protein that is involved in both purine biosynthesis and in the formation of adenosine monophosphate (AMP) from inosine monophosphate. Expressed ubiquitously, ADSL catalyzes two key reactions in AMP biosynthesis, namely the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and the subsequent removal of fumarate from adenylosuccinate to yield AMP. Defects in the gene encoding ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency), an autosomal recessive disorder characterized by epilepsy, growth retardation and muscular wasting. Multiple isoforms of ADSL exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenylosuccinase; ADSL; alternatively spliced; unnamed protein product
Gene Aliases: AMPS; ASASE; ASL
UniProt ID: (Human) P30566
Entrez Gene ID: (Human) 158
response to hypoxia
purine nucleotide biosynthetic process
AMP biosynthetic process
GMP biosynthetic process
'de novo' IMP biosynthetic process
response to nutrient
aerobic respiration
purine ribonucleotide biosynthetic process
response to muscle activity
response to starvation
'de novo' AMP biosynthetic process
AMP salvage
'de novo' XMP biosynthetic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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