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Invitrogen
RFWD2 Recombinant Rabbit Monoclonal Antibody (5A0S9)
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FIGURE: 1 / 1
RFWD2 Antibody (MA5-57925) in WB
Western blot analysis of RFWD2 in Rat spleen lysates (25 µg/lane). Samples were incubated overnight at 4°C with RFWD2 Recombinant Monoclonal Antibody (Product # MA5-57926) using a dilution of 1:1, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 30 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57925
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5A0S9
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human COP1/RFWD2 (NP_071902.2).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.16 mg/mL
Amount
23.2 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: YYDLRNTKQP IMVFKGHRKA VSYAKFVSGE EIVSASTDSQ LKLWNVGKPY CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV
Target Information
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: constitutive photomorphogenesis protein 1 homolog; constitutive photomorphogenic protein 1; COP1D; E3 ubiquitin protein ligase; E3 ubiquitin-protein ligase RFWD2; hCOP1 d24; mCOP1; putative ubiquitin ligase COP1; ring finger and WD repeat domain 2; ring finger and WD repeat domain 2, E3 ubiquitin protein ligase; RING finger and WD repeat domain protein 2; RING finger protein 200; RING-finger protein; RING-type E3 ubiquitin transferase RFWD2; unnamed protein product
Gene Aliases: CFAP78; FAP78; RFWD2; RGD1304773; RNF200
UniProt ID: (Human) Q8NHY2, (Mouse) Q9R1A8
Entrez Gene ID: (Human) 64326, (Mouse) 26374, (Rat) 360860
ubiquitin-dependent protein catabolic process
response to ionizing radiation
protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of fatty acid biosynthetic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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