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Invitrogen
Cyclophilin A Recombinant Rabbit Monoclonal Antibody (4L4U4)
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FIGURE: 1 / 1
Cyclophilin A Antibody (MA5-57931) in WB
Western blot analysis of Cyclophilin A in Hep G2, HeLa, and rat spleen (25 µg/lane). Samples were incubated overnight at 4°C with Cyclophilin A Recombinant Monoclonal Antibody (Product # MA5-57932) using a dilution of 1:2, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 20 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57931
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4L4U4
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
20 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: RIIPGFMCQG GDFTRHNGTG GKSIYGEKFE DENFILKHTG PGILSMANAG PNTNGSQFFI CTAKTEWLDG KHVVFGKVKE GMNIVEAMER FGSRNGKTSK
Target Information
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cyclophilin; cyclophilin A; cyclosporin A-binding protein; CyP A; EC 5.2.1.8; epididymis secretory sperm binding protein Li 69p; HGNC:9253; MGC117158; MGC12404; MGC23397; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase; PPIase A; rotamase; rotamase A; T cell cyclophilin; unnamed protein product
Gene Aliases: Cphn; CYCA; CyP-18; CyP-A; CYPA; CYPH; HEL-S-69p; p1B15; p31; SP18
UniProt ID: (Human) P62937, (Rat) P10111, (Mouse) P17742
Entrez Gene ID: (Human) 5478, (Rat) 25518, (Mouse) 268373
protein peptidyl-prolyl isomerization
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
protein folding
negative regulation of protein kinase activity
apoptotic process
viral release from host cell
platelet activation
neutrophil chemotaxis
leukocyte chemotaxis
activation of protein kinase B activity
negative regulation of stress-activated MAPK cascade
lipid particle organization
cellular response to oxidative stress
endothelial cell activation
positive regulation of MAPK cascade
regulation of viral genome replication
positive regulation of viral genome replication
positive regulation of protein secretion
cell adhesion molecule production
negative regulation of protein K48-linked ubiquitination
platelet aggregation
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
negative regulation of viral life cycle
regulation of apoptotic signaling pathway
neuron differentiation
negative regulation of calcineurin-mediated signaling
chylomicron assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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