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GRP94 Recombinant Rabbit Monoclonal Antibody (9U3N5)
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FIGURE: 1 / 1
GRP94 Antibody (MA5-57943) in WB
Western blot analysis of GRP94 in MCF7, HeLa, and PC-12 (25 µg/lane). Samples were incubated overnight at 4°C with GRP94 Recombinant Monoclonal Antibody (Product # MA5-57944) using a dilution of 1:2, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 60 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57943
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9U3N5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 616-715 of human Grp94 (NP_003290.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.57 mg/mL
Amount
31.4 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: EPLLNWMKDK ALKDKIEKAV VSQRLTESPC ALVASQYGWS GNMERIMKAQ AYQTGKDIST NYYASQKKTF EINPRHPLIR DMLRRIKEDE DDKTVLDLAV
Target Information
GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 94 kDa glucose-regulated protein; endoplasmic reticulum resident protein 99; endoplasmic reticulum transmembrane protein precursor; endoplasmin; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; GRP-94; GRP94; heat shock protein 90 kDa beta member 1; heat shock protein 90, beta, member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; heat shock protein family C member 4; polymorphic tumor rejection antigen 1; polymorphic tumor rejection antigen precursor; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; tumor rejection antigen 1; tumor rejection antigen gp96; unnamed protein product
Gene Aliases: ECGP; endoplasmin; ERp99; GP96; GRP94; HEL-S-125m; HEL35; TA-3; Targ2; Tra-1; TRA1
UniProt ID: (Human) P14625, (Mouse) P08113, (Rat) Q66HD0
Entrez Gene ID: (Human) 7184, (Mouse) 22027, (Rat) 362862
RNA binding
protein phosphatase inhibitor activity
calcium ion binding
protein binding
ATP binding
ATPase activity
protein phosphatase binding
AP-3 adaptor complex binding
protein binding involved in protein folding
low-density lipoprotein particle receptor binding
ATP-dependent protein folding chaperone
response to hypoxia
protein folding
response to hormone
insulin processing
positive regulation of Wnt signaling pathway
retrograde protein transport, ER to cytosol
actin rod assembly
positive regulation of toll-like receptor signaling pathway
ERAD pathway
negative regulation of apoptotic process
cellular response to manganese ion
cellular response to ATP
protein localization to plasma membrane
protein transport
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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