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PMCA1 ATPase Recombinant Rabbit Monoclonal Antibody (1X7X6)
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FIGURE: 1 / 1
PMCA1 ATPase Antibody (MA5-58103) in WB
Western blot analysis of PMCA1 ATPase in Mouse liver lysates (25 µg/lane). Samples were incubated overnight at 4°C with PMCA1 ATPase Recombinant Monoclonal Antibody (Product # MA5-58104) using a dilution of 1:1, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 45 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-58103
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1X7X6
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP2B1 (NP_001673.2).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.96 mg/mL
Amount
39.2 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: MGDMANNSVA YSGVKNSLKE ANHDGDFGIT LAELRALMEL RSTDALRKIQ ESYGDVYGIC TKLKTSPNEG LSGNPADLER REAVFGKNFI PPKKPKTFLQ
Target Information
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, Ca++ transporting, plasma membrane 1; calcium pump; plasma membrane calcium ATPase; plasma membrane calcium pump; plasma membrane calcium-ATPase isoform 1 alternative splice variant k at splice site B and splice variant b at splice site C; PMCA1; PMCA1kb; unnamed protein product
Gene Aliases: 2810442I22Rik; E130111D10Rik; MRD66; PMCA1; Pmca1a; Pmca1b; Pmca1c; PMCA1kb
UniProt ID: (Human) P20020, (Mouse) G5E829, (Rat) P11505
Entrez Gene ID: (Human) 490, (Mouse) 67972, (Rat) 29598
negative regulation of cytokine production
regulation of vascular smooth muscle contraction
calcium ion transport
cellular calcium ion homeostasis
regulation of blood pressure
positive regulation of bone mineralization
regulation of cytosolic calcium ion concentration
negative regulation of cytosolic calcium ion concentration
positive regulation of calcium ion transport
calcium ion transmembrane transport
regulation of cellular response to insulin stimulus
calcium ion export
calcium ion export from cell
ion transmembrane transport
regulation of cardiac conduction
neural retina development
cellular response to vitamin D
cellular response to corticosterone stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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