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ENO3 Recombinant Rabbit Monoclonal Antibody (2F9I8)
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FIGURE: 1 / 1
ENO3 Antibody (MA5-58189) in WB
Western blot analysis of ENO3 in HeLa, mouse heart, and rat heart (25 µg/lane). Samples were incubated overnight at 4°C with ENO3 Recombinant Monoclonal Antibody (Product # MA5-58190) using a dilution of 1:3, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 30 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-58189
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2F9I8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ENO3 (NP_001967.3).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.24 mg/mL
Amount
24.8 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN NTLGPALLQK KLSVVDQEKV DKFMIELDGT
Target Information
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; Eno3; enob; enolase 3 (beta, muscle); enolase 3, beta, muscle; muscle enriched enolase; Muscle specific enolase (beta beta enolose) (two splicing products); muscle-specific enolase; muscle-specific enolase beta subunit (EC 4.2.1.11); skeletal muscle enolase; unnamed protein product
Gene Aliases: BBE; Eno-3; GSD13; MSE
UniProt ID: (Human) P13929, (Rat) P15429, (Mouse) P21550
Entrez Gene ID: (Human) 2027, (Rat) 25438, (Mouse) 13808
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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