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Invitrogen
IL-1 alpha Recombinant Rabbit Monoclonal Antibody (9B10I4)
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FIGURE: 1 / 1
IL-1 alpha Antibody (MA5-58191) in WB
Western blot analysis of IL-1 alpha in Recombinant Human IL-1 alpha Protein lysates (5 and 10 ng/lane). Samples were incubated overnight at 4°C with IL-1 alpha Recombinant Monoclonal Antibody (Product # MA5-58192) using a dilution of 1:2, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 90 seconds.
Product Details
MA5-58191
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9B10I4
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 113-271 of human IL-1 alpha (NP_000566.3).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
20 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA
Target Information
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA8; Hematopoietin-1; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1A; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin-17A; Interleukin1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha; RP23-160G19.8; unnamed protein product
Gene Aliases: IL-1 alpha; IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1
UniProt ID: (Human) P01583
Entrez Gene ID: (Human) 3552
fever generation
positive regulation of cytokine production
connective tissue replacement involved in inflammatory response wound healing
cellular sodium ion homeostasis
apoptotic process
response to stress
inflammatory response
immune response
negative regulation of cell proliferation
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
cytokine-mediated signaling pathway
positive regulation of interleukin-2 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of immature T cell proliferation in thymus
cellular response to heat
ectopic germ cell programmed cell death
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of angiogenesis
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
response to copper ion
positive regulation of protein secretion
positive regulation of cell division
regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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