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Invitrogen
Laminin gamma-1 Recombinant Rabbit Monoclonal Antibody (7B2C2)
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FIGURE: 1 / 1
Laminin gamma-1 Antibody (MA5-58203) in WB
Western blot analysis of Laminin gamma-1 in mouse heart and rat heart (25 µg/lane). Samples were incubated overnight at 4°C with Laminin gamma-1 Recombinant Monoclonal Antibody (Product # MA5-58204) using a dilution of 1:3, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 90 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-58203
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7B2C2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1030-1275 of human LAMC1 (NP_002284.3).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.24 mg/mL
Amount
44.8 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: ECPACYRLVK DKVADHRVKL QELESLIANL GTGDEMVTDQ AFEDRLKEAE REVMDLLREA QDVKDVDQNL MDRLQRVNNT LSSQISRLQN IRNTIEETGN LAEQARAHVE NTERLIEIAS RELEKAKVAA ANVSVTQPES TGDPNNMTLL AEEARKLAER HKQEADDIVR VAKTANDTST EAYNLLLRTL AGENQTAFEI EELNRKYEQA KNISQDLEKQ AARVHEEAKR AGDKAVEIYA SVAQLS
Target Information
Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: laminin B2 chain; laminin B2 precursor; laminin, gamma 1; laminin, gamma 1 (formerly LAMB2); laminin-1 subunit gamma; laminin-10 subunit gamma; laminin-11 subunit gamma; laminin-2 subunit gamma; laminin-3 subunit gamma; laminin-4 subunit gamma; laminin-6 subunit gamma; laminin-7 subunit gamma; laminin-8 subunit gamma; laminin-9 subunit gamma; S-LAM gamma; S-laminin subunit gamma; unnamed protein product
Gene Aliases: B2e; LAMB2
UniProt ID: (Human) P11047
Entrez Gene ID: (Human) 3915, (Mouse) 226519, (Rat) 117036
chromatin organization
cell adhesion
signal transduction
axon guidance
organ morphogenesis
tissue development
gene expression
cell migration
extracellular matrix disassembly
hair follicle morphogenesis
neuron projection development
hemidesmosome assembly
substrate adhesion-dependent cell spreading
hair cell differentiation
positive regulation of cell adhesion
tissue morphogenesis
positive regulation of muscle cell differentiation
protein-containing complex assembly
hair follicle cell proliferation
regulation of basement membrane organization
positive regulation of skeletal muscle acetylcholine-gated channel clustering
positive regulation of integrin-mediated signaling pathway
endoderm development
regulation of cell adhesion
regulation of cell migration
maintenance of blood-brain barrier
regulation of embryonic development
positive regulation of epithelial cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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