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Invitrogen
PI16 Recombinant Rabbit Monoclonal Antibody (9Z5V7)
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FIGURE: 1 / 1
PI16 Antibody (MA5-58211) in WB
Western blot analysis of PI16 in wild type (WT) and 293T cell lysates transfected with CD364/PI16 (20 µg/lane). Samples were incubated overnight at 4°C with PI16 Recombinant Monoclonal Antibody (Product # MA5-58212) using a dilution of 1:1, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 10 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-58211
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9Z5V7
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-210 of mouse CD364/PI16 (NP_076223.3).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.13 mg/mL
Amount
22.6 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: LTEDEKQTMV DLHNQYRAQV SPPASDMLQM RWDDELAAFA KAYAQKCVWG HNKERGRRGE NLFAITDEGM DVPLAVGNWH EEHEYYNFST ATCDPNQMCG HYTQVVWSKT ERIGCGSHFC ETLQGVEEAN IHLLVCNYEP PGNVKGRKPY QEGTPCSQCP LGYSCENSLC EPMRNPEKAQ D
Target Information
Peptidase inhibitor 16 (PI16), also known as CRISP-9 or PSPBP, is a member of the cysteine-rich secretory protein (CRISP) family and plays a significant role in various biological processes. This protein consists of 436 amino acids and features three N-linked glycosylation motifs, suggesting its localization to the plasma membrane through a GPI anchor. PI16 has been implicated as a binding protein for prostate secretory protein of 94 amino acids (PSP94), which reflects its potential involvement in reproductive biology. Recent findings highlight PI16's expression in fibroblasts, particularly marking a precursor fibroblast cell state, which is critical in fibroblast lineage development. This expression is heightened in certain pathological conditions, such as neuropathic pain and inflammatory arthritis. In neuropathic pain models such as the spared nerve injury (SNI) model, elevated PI16 levels are noted in fibroblasts within the dorsal root ganglia (DRG) and sciatic nerve, but not in neurons or glia. Interestingly, mice lacking PI16 are protected against neuropathic pain, indicating its role in enhancing pain signaling. Conversely, in rheumatoid arthritis (RA), PI16's overexpression in peripheral blood and synovial fluid correlates with worsened arthritis severity, partly by suppressing the expression of the regulatory T cell marker Foxp3. This complex role of PI16, affecting various signaling pathways and cellular states, underscores its potential as a therapeutic target in pain and inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cysteine-rich protease inhibitor; protease inhibitor 16
Gene Aliases: 1200009H11Rik; Cripi; PI-16
UniProt ID: (Mouse) Q9ET66
Entrez Gene ID: (Mouse) 74116
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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