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Invitrogen
TRAF1 Recombinant Rabbit Monoclonal Antibody (PSH13-88)
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FIGURE: 1 / 8
TRAF1 Antibody (MA562930) in WB
Western blot was performed using TRAF1 Recombinant Rabbit Monoclonal Antibody (PSH13-88) (Product # MA562930) and a ~50 kDa band corresponding to TRAF1 was observed across cell lines and tissues tested. Whole cell (20 µg lysate) and tissue (40 µg lysate) extracts of Raji (Lane 1), HEK-293(negative) (Lane 2), Ramos (Lane 3), Daudi (Lane 4) and rat spleen (Lane 5) were electrophoresed using 4-20% SDS-PAGE gel. Resolved proteins were transferred onto a PVDF membrane. The blot was blocked with 5% NFDM/TBST for 1 hour at room temperature, then ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA562930
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH13-88
Immunogen
Recombinant protein within human TRAF1 aa 1-200.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive Control: Raji cell lysate, Ramos cell lysate, Daudi cell lysate, Rat spleen tissue lysate.
Tissue Specificity: Tissue enhanced (lymphoid)
Subcellular Location: Cytoplasm, cytosol.
Sequence Similarities: 72% Mouse/Rat
Predicted band size: 46 kDa
Target Information
The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. Three transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Epstein-Bar virus-induced protein 6; unnamed protein product
Gene Aliases: EBI6; MGC:10353
UniProt ID: (Human) Q13077
Entrez Gene ID: (Human) 7185, (Rat) 687813
signal transduction
tumor necrosis factor-mediated signaling pathway
regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
protein-containing complex assembly
positive regulation of pyroptotic inflammatory response
negative regulation of NIK/NF-kappaB signaling
regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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