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Invitrogen
Phospho-Cofilin (Ser3) Recombinant Rabbit Monoclonal Antibody (2P6N0)
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FIGURE: 1 / 2
Phospho-Cofilin (Ser3) Antibody (MA575820) in WB
Western blot analysis of Phospho-Cofilin (Ser3) in 293T, NIH/3T3 (30 μg per lane). Samples were blocked with 3% nonfat dry milk in TBST, incubated with monoclonal Phospho-Cofilin (Ser3) (Product # MA575820) antibody at a dilution of 1:5,000 (overnight at 4°C), followed by HRP-conjugated Goat anti-Rabbit IgG (H+L) (1:10,000) and ECL. Exposure time: 5s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA575820
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2P6N0
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 18 kDa phosphoprotein; CFL-1; COF1; cofilin 1 (non-muscle); cofilin 1, non-muscle; Cofilin1; epididymis secretory protein Li 15; n-cofilin; p18; unnamed protein product
Gene Aliases: CFL; Cof; cofilin; HEL-S-15
UniProt ID: (Human) P23528, (Rat) P45592, (Mouse) P18760
Entrez Gene ID: (Human) 1072, (Rat) 29271, (Mouse) 12631
mitotic cytokinesis
protein import into nucleus
negative regulation of cell adhesion
Rho protein signal transduction
actin polymerization or depolymerization
response to virus
positive regulation of lamellipodium assembly
negative regulation of lamellipodium assembly
response to activity
hippocampus development
actin cytoskeleton organization
actin filament depolymerization
actin filament fragmentation
positive regulation of cell growth
negative regulation of actin filament depolymerization
positive regulation of actin filament depolymerization
positive regulation of synaptic plasticity
negative regulation of actin filament bundle assembly
positive regulation of embryonic development
negative regulation of apoptotic process
positive regulation by host of viral process
positive regulation of proteolysis
modulation of synaptic transmission
actin filament severing
establishment of spindle localization
negative regulation of unidimensional cell growth
positive regulation of focal adhesion assembly
positive regulation of dendritic spine development
regulation of dendritic spine morphogenesis
cellular response to hydrogen peroxide
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to ether
cellular response to epidermal growth factor stimulus
modification of postsynaptic actin cytoskeleton
negative regulation of dendritic spine maintenance
positive regulation of protein localization to cell leading edge
negative regulation of postsynaptic density organization
cellular response to insulin-like growth factor stimulus
negative regulation of cell motility
positive regulation of cell motility
positive regulation of establishment of cell polarity regulating cell shape
positive regulation of barbed-end actin filament capping
neural crest cell migration
neural fold formation
protein phosphorylation
actin filament organization
establishment of cell polarity
cell projection organization
response to amino acid
negative regulation of cell size
cell motility
regulation of N-methyl-D-aspartate selective glutamate receptor activity
cytoskeleton organization
regulation of cell morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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