Nigericin, Free Acid

Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K⁺/H⁺ ionophore nigericin, which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K⁺.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›