Newport Green™ DCF Diacetate, cell permeant

The Newport Green™ DCF indicator has moderate zinc-binding affinity (K_d for Zn²⁺ ∼1 μM) but is essentially insensitive to Ca²⁺ (Kd for Ca²⁺ >100 μM), making this a valuable probe for detecting Zn²⁺ influx into neurons through voltage- or glutamate-gated channels. When used alongside dyes with dual Ca²⁺ / Zn²⁺ sensitivity such as fura-2 and mag-fura-2, Newport Green™ DCF provides confirmation that changes in Zn²⁺ levels, and not Ca²⁺ or Mg²⁺, are being detected. The response of Newport Green™ DCF to transition metals, including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd, has been characterized.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Newport Green™ PDX has a higher Zn²⁺ dissociation constant (K_d for Zn²⁺ ∼30 μM) and a larger Zn²⁺-free to Zn²⁺-saturated fluorescence intensity increase than the Newport Green™ DCF indicators.

Fluorescent Zinc Indicators Specifications:
• Label (Ex/Em): Newport Green™ DCF (∼505/535 nm)
• K_d (Zn²⁺) (in buffer): ∼1 μM
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding the dissolved indicator to medium containing cells

Find Fluorescent Indicators for Zinc and Other Metal Ions
We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more. Review Fluorescent Indicators for Zn²⁺ and Other Metal Ions—Section 19.7 in the Molecular Probes™ Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.