Chimpanzee Fab fragments and a derived humanized immunoglobulin G1 antibody that efficiently cross-neutralize dengue type 1 and type 2 viruses.
AuthorsGoncalvez AP; Men R; Wernly C; Purcell RH; Lai CJ
JournalJournal of Virology
PubMed ID
Passive immunization with monoclonal antibodies from humans or nonhuman primates represents an attractive alternative to vaccines for prevention of illness caused by dengue viruses (DENV) and other flaviviruses, including the West Nile virus. In a previous study, repertoire cloning to recover Fab fragments from bone marrow mRNA of chimpanzees infected ... More
Molecular genetics of tetrahydrobiopterin (BH4) deficiency in the Maltese population.
Deficient activity of the Dihydropteridine Reductase enzyme (DHPR; EC 1.5.1.34; OMIM 261630) is due to mutations in the Quinoid Dihydropteridine Reductase gene on 4p15.3 (QDPR; RefSeq NM_000320). It results in defective recycling of tetrahydrobiopterin (BH(4)) and homozygotes have a rare form of atypical Hyperphenylalaninaemia and Phenylketonuria (aPKU). The heterozygote frequency ... More
A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast.
AuthorsEisenkolb M; Zenzmaier C; Leitner E; Schneiter R
JournalMolecular Biology of the Cell
PubMed ID
Fungal sphingolipids contain ceramide with a very-long-chain fatty acid (C26). To investigate the physiological significance of the C26-substitution on this lipid, we performed a screen for mutants that are synthetically lethal with ELO3. Elo3p is a component of the ER-associated fatty acid elongase and is required for the final elongation ... More
Procollagen with skipping of alpha 1(I) exon 41 has lower binding affinity for alpha 1(I) C-telopeptide, impaired in vitro fibrillogenesis, and altered fibril morphology.
Previous in vitro data on type I collagen self-assembly into fibrils suggested that the amino acid 776-796 region of the alpha1(I) chain is crucial for fibril formation because it serves as the recognition site for the telopeptide of a docking collagen monomer. We used a natural collagen mutation with a ... More
Vibrio fischeri LuxS and AinS: comparative study of two signal synthases.
AuthorsLupp C; Ruby EG
JournalJournal of Bacteriology
PubMed ID
Vibrio fischeri possesses two acyl-homoserine lactone quorum-sensing systems, ain and lux, both of which are involved in the regulation of luminescence gene expression and are required for persistent colonization of the squid host, Euprymna scolopes. We have previously demonstrated that the ain system induces luminescence at cell densities that precede ... More
Activating the synthesis of progerin, the mutant prelamin A in Hutchinson-Gilford progeria syndrome, with antisense oligonucleotides
Hutchinson-Gilford progeria syndrome (HGPS) is caused by point mutations that increase utilization of an alternate splice donor site in exon 11 of LMNA (the gene encoding lamin C and prelamin A). The alternate splicing reduces transcripts for wild-type prelamin A and increases transcripts for a truncated prelamin A (progerin). Here, ... More
Human biliverdin reductase is a leucine zipper-like DNA-binding protein and functions in transcriptional activation of heme oxygenase-1 by oxidative stress.
AuthorsAhmad Z; Salim M; Maines MD
JournalThe Journal of Biological Chemistry
PubMed ID
Human biliverdin reductase (hBVR) is a serine/threonine kinase that catalyzes reduction of the heme oxygenase (HO) activity product, biliverdin, to bilirubin. A domain of biliverdin reductase (BVR) has primary structural features that resemble leucine zipper proteins. A heptad repeat of five leucines (L(1)--L(5)), a basic domain, and a conserved alanine ... More
The unique cyanobacterial protein OpcA is an allosteric effector of glucose-6-phosphate dehydrogenase in Nostoc punctiforme ATCC 29133.
AuthorsHagen KD; Meeks JC
JournalThe Journal of Biological Chemistry
PubMed ID
Glucose-6-phosphate dehydrogenase (G6PD), encoded by zwf, is essential for nitrogen fixation and dark heterotrophic growth of the cyanobacterium Nostoc punctiforme ATCC 29133. In N. punctiforme, zwf is part of a four-gene operon transcribed in the order fbp-tal-zwf-opcA. Genetic analyses indicated that opcA is required for G6PD activity. To define the ... More
Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine.
AuthorsChen H; Ordog T; Chen J; Young DL; Bardsley MR; Redelman D; Ward SM; Sanders KM
JournalPhysiological Genomics
PubMed ID
Interstitial cells of Cajal (ICC) have important functions in regulation of motor activity in the gastrointestinal tract. In murine small intestine, ICC are gathered in the regions of the myenteric plexus (ICC-MY) and the deep muscular plexus (ICC-DMP). These two classes of ICC have different physiological functions. ICC-MY are pacemaker ... More
ClC-3 is a fundamental molecular component of volume-sensitive outwardly rectifying Cl- channels and volume regulation in HeLa cells and Xenopus laevis oocytes.
AuthorsHermoso M; Satterwhite CM; Andrade YN; Hidalgo J; Wilson SM; Horowitz B; Hume JR
JournalThe Journal of Biological Chemistry
PubMed ID
Volume-sensitive osmolyte and anion channels (VSOACs) are activated upon cell swelling in most vertebrate cells. Native VSOACs are believed to be a major pathway for regulatory volume decrease (RVD) through efflux of chloride and organic osmolytes. ClC-3 has been proposed to encode native VSOACs in Xenopus laevis oocytes and in ... More
Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement.
AuthorsCai X; Li M; Vrana J; Schaller MD
JournalMolecular and Cellular Biology
PubMed ID
Paxillin is a 68-kDa focal adhesion-associated protein that plays an important role in controlling cell spreading and migration. Phosphorylation of paxillin regulates its biological activity and thus has warranted investigation. Serine 126 and serine 130 were previously identified as two major extracellular signal-regulated kinase (ERK)-dependent phosphorylation sites in Raf-transformed fibroblasts. ... More
Succinate secreted by Trypanosoma brucei is produced by a novel and unique glycosomal enzyme, NADH-dependent fumarate reductase.
AuthorsBesteiro S; Biran M; Biteau N; Coustou V; Baltz T; Canioni P; Bringaud F
JournalThe Journal of Biological Chemistry
PubMed ID
In all trypanosomatids, including Trypanosoma brucei, glycolysis takes place in peroxisome-like organelles called glycosomes. These are closed compartments wherein the energy and redox (NAD(+)/NADH) balances need to be maintained. We have characterized a T. brucei gene called FRDg encoding a protein 35% identical to Saccharomyces cerevisiae fumarate reductases. Microsequencing of ... More
Exo-proofreading, a versatile SNP scoring technology.
AuthorsCahill P; Bakis M; Hurley J; Kamath V; Nielsen W; Weymouth D; Dupuis J; Doucette-Stamm L; Smith DR
JournalGenome Research
PubMed ID
We report the validation of a new assay for typing single nucleotide polymorphisms (SNPs) that takes advantage of the 3'-to-5' exonuclease proofreading activity of many DNA polymerases. The assay uses one or more primers labeled on the 3' nucleotide base, and can be implemented in a variety of formats including ... More
Nocturnal production of endospores in natural populations of epulopiscium-like surgeonfish symbionts.
AuthorsFlint JF; Drzymalski D; Montgomery WL; Southam G; Angert ER
JournalJournal of Bacteriology
PubMed ID
Prior studies have described a morphologically diverse group of intestinal microorganisms associated with surgeonfish. Despite their diversity of form, 16S rRNA gene surveys and fluorescent in situ hybridizations indicate that these bacteria are low-G+C gram-positive bacteria related to Epulopiscium spp. Many of these bacteria exhibit an unusual mode of reproduction, ... More
Clitocypin, a new type of cysteine proteinase inhibitor from fruit bodies of mushroom clitocybe nebularis.
AuthorsBrzin J; Rogelj B; Popovic T; Strukelj B; Ritonja A
JournalThe Journal of Biological Chemistry
PubMed ID
A novel inhibitor of cysteine proteinases has been isolated from fruit bodies of a mushroom Clitocybe nebularis. The inhibitor was purified to homogeneity by affinity chromatography and gel filtration, followed by reverse-phase high pressure liquid chromatography. The active inhibitor has an apparent molecular mass of about 34 kDa by gel ... More
Cloning and characterization of four Anopheles gambiae serpin isoforms, differentially induced in the midgut by Plasmodium berghei invasion.
AuthorsDanielli A; Kafatos FC; Loukeris TG
JournalThe Journal of Biological Chemistry
PubMed ID
The genomic locus SRPN10 of the malaria vector Anopheles gambiae codes for four alternatively spliced serine protease inhibitors of the serpin superfamily. The four 40- to 42-kDa isoforms differ only at their C terminus, which bears the reactive site loop, and exhibit protein sequence similarity with other insect serpins and ... More
A Glu-496 to Ala polymorphism leads to loss of function of the human P2X7 receptor.
The P2X(7) receptor is a ligand-gated cation-selective channel that mediates ATP-induced apoptosis of cells of the immune system. We and others have shown that P2X(7) is nonfunctional both in lymphocytes and monocytes from some subjects. To study a possible genetic basis we sequenced DNA coding for the carboxyl-terminal tail of ... More
DBC2, a candidate for a tumor suppressor gene involved in breast cancer.
AuthorsHamaguchi M; Meth JL; von Klitzing C; Wei W; Esposito D; Rodgers L; Walsh T; Welcsh P; King MC; Wigler MH
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID
A previously uncharacterized gene, DBC2 (deleted in breast cancer), was cloned from a homozygously deleted region at human chromosome 8p21. DBC2 contains a highly conserved RAS domain and two putative protein interacting domains. Our analyses indicate that DBC2 is the best candidate tumor suppressor gene from this region. It lies ... More
The N termini of focal adhesion kinase family members regulate substrate phosphorylation, localization, and cell morphology.
AuthorsDunty JM; Schaller MD
JournalThe Journal of Biological Chemistry
PubMed ID
The focal adhesion kinase (FAK) and cell adhesion kinase beta (CAKbeta, PYK2, CADTK, RAFTK) are highly homologous FAK family members, yet clearly have unique roles in the cell. Comparative analyses of FAK and CAKbeta have revealed intriguing differences in their activities. These differences were investigated further through the characterization of ... More
Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest.
AuthorsBennin DA; Don AS; Brake T; McKenzie JL; Rosenbaum H; Ortiz L; DePaoli-Roach AA; Horne MC
JournalThe Journal of Biological Chemistry
PubMed ID
Cyclin G2, together with cyclin G1 and cyclin I, defines a novel cyclin family expressed in terminally differentiated tissues including brain and muscle. Cyclin G2 expression is up-regulated as cells undergo cell cycle arrest or apoptosis in response to inhibitory stimuli independent of p53 (Horne, M., Donaldson, K., Goolsby, G., ... More
Genome-wide expression analyses of Campylobacter jejuni NCTC11168 reveals coordinate regulation of motility and virulence by flhA.
AuthorsCarrillo CD; Taboada E; Nash JH; Lanthier P; Kelly J; Lau PC; Verhulp R; Mykytczuk O; Sy J; Findlay WA; Amoako K; Gomis S; Willson P; Austin JW; Potter A; Babiuk L; Allan B; Szymanski CM
JournalThe Journal of Biological Chemistry
PubMed ID
We examined two variants of the genome-sequenced strain, Campylobacter jejuni NCTC11168, which show marked differences in their virulence properties including colonization of poultry, invasion of Caco-2 cells, and motility. Transcript profiles obtained from whole genome DNA microarrays and proteome analyses demonstrated that these differences are reflected in late flagellar structural ... More
Coxsackie B4 virus infection of beta cells and natural killer cell insulitis in recent-onset type 1 diabetic patients.
AuthorsDotta F; Censini S; van Halteren AG; Marselli L; Masini M; Dionisi S; Mosca F; Boggi U; Muda AO; Prato SD; Elliott JF; Covacci A; Rappuoli R; Roep BO; Marchetti P
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID
Type 1 diabetes is characterized by T cell-mediated autoimmune destruction of pancreatic beta cells. Several studies have suggested an association between Coxsackie enterovirus seroconversion and onset of disease. However, a direct link between beta cell viral infection and islet inflammation has not been established. We analyzed pancreatic tissue from six ... More
An Arg307 to Gln polymorphism within the ATP-binding site causes loss of function of the human P2X7 receptor.
The P2X(7) receptor is a ligand-gated channel that is highly expressed on mononuclear cells of the immune system and that mediates ATP-induced apoptosis. Wide variations in the function of the P2X receptor have been observed, explained in part by (7)loss-of-function polymorphisms that change Glu(496) to Ala (E496A) and Ile(568) to ... More
Expression cloning and characterization of the C28 acyltransferase of lipid A biosynthesis in Rhizobium leguminosarum.
AuthorsBasu SS; Karbarz MJ; Raetz CR
JournalThe Journal of Biological Chemistry
PubMed ID
An unusual feature of lipid A from plant endosymbionts of the Rhizobiaceae family is the presence of a 27-hydroxyoctacosanoic acid (C28) moiety. An enzyme that incorporates this acyl chain is present in extracts of Rhizobium leguminosarum, Rhizobium etli, and Sinorhizobium meliloti but not Escherichia coli. The enzyme transfers 27-hydroxyoctacosanate from ... More
Impairment of the TFIIH-associated CDK-activating kinase selectively affects cell cycle-regulated gene expression in fission yeast.
AuthorsLee KM; Miklos I; Du H; Watt S; Szilagyi Z; Saiz JE; Madabhushi R; Penkett CJ; Sipiczki M; Bahler J; Fisher RP
JournalMolecular Biology of the Cell
PubMed ID
The fission yeast Mcs6-Mcs2-Pmh1 complex, homologous to metazoan Cdk7-cyclin H-Mat1, has dual functions in cell division and transcription: as a partially redundant cyclin-dependent kinase (CDK)-activating kinase (CAK) that phosphorylates the major cell cycle CDK, Cdc2, on Thr-167; and as the RNA polymerase (Pol) II carboxyl-terminal domain (CTD) kinase associated with ... More
Neurite extension in central neurons: a novel role for the receptor tyrosine kinases Ror1 and Ror2.
AuthorsPaganoni S; Ferreira A
JournalJournal of Cell Science
PubMed ID
Neurite elongation and branching are key cellular events during brain development as they underlie the formation of a properly wired neuronal network. Here we report that the receptor tyrosine kinases Ror1 and Ror2 modulate the growth of neurites as well as their branching pattern in hippocampal neurons. Upon Ror1 or ... More
Mitochondrial transcription factor A induction by redox activation of nuclear respiratory factor 1.
AuthorsPiantadosi CA; Suliman HB
JournalThe Journal of Biological Chemistry
PubMed ID
The nuclear expression of mitochondrial transcription factor A (Tfam), which is required for mitochondrial DNA (mtDNA) transcription and replication, must be linked to cellular energy needs. Because respiration generates reactive oxygen species as a side-product, we tested the idea that reactive oxygen species regulate Tfam expression through phosphorylation of nuclear ... More
Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis.
AuthorsYeh HC; Ho YP; Shih IeM; Wang TH
JournalNucleic Acids Research
PubMed ID
This report describes a new genotyping method capable of detecting low-abundant point mutations in a homogeneous, separation-free format. The method is based on integration of oligonucleotide ligation with a semiconductor quantum dot (QD)-mediated two-color fluorescence coincidence detection scheme. Surface-functionalized QDs are used to capture fluorophore-labeled ligation products, forming QD-oligonucleotide nanoassemblies. ... More
Differential expression of interferon (IFN) regulatory factors and IFN-stimulated genes at early times after West Nile virus infection of mouse embryo fibroblasts.
AuthorsScherbik SV; Stockman BM; Brinton MA
JournalJournal of Virology
PubMed ID
Although lineage I West Nile virus (WNV) strain Eg101 induced beta interferon (IFN-beta) production as early as 12 h after infection in primary mouse embryo fibroblasts and did not inhibit the JAK-STAT signaling pathway, it was still able to replicate efficiently. To gain insights about possible viral countermeasures used by ... More
A sex-specific role of type VII adenylyl cyclase in depression.
AuthorsHundesa A; Maluquer de Motes C; Bofill-Mas S; Albinana-Gimenez N; Girones R
JournalThe Journal of Neuroscience : the Official Journal of the Society for Neuroscience
PubMed ID
Major depression represents a complex mental disorder. The identification of biological markers that define subtypes of major depressive disorder would greatly facilitate appropriate medical treatments, as well as provide insight into etiology. Reduced activity of the cAMP signaling system has been implicated in the etiology of major depression. Previous work ... More
DNA microarray analysis of vitamin D-induced gene expression in a human colon carcinoma cell line.
AuthorsWood RJ; Tchack L; Angelo G; Pratt RE; Sonna LA
JournalPhysiological Genomics
PubMed ID
The full extent to which 1,25-dihydroxyvitamin D(3) affects gene expression in human intestinal epithelial cells is unknown. We used oligonucleotide arrays to catalog vitamin D-induced changes in gene expression in Caco-2 cells, a human colon carcinoma cell line. Five paired sets of Caco-2 cell cultures were subjected to either control ... More
Cux2 (Cutl2) integrates neural progenitor development with cell-cycle progression during spinal cord neurogenesis.
AuthorsIulianella A; Sharma M; Durnin M; Vanden Heuvel GB; Trainor PA
JournalDevelopment (Cambridge, England)
PubMed ID
Neurogenesis requires the coordination of neural progenitor proliferation and differentiation with cell-cycle regulation. However, the mechanisms coordinating these distinct cellular activities are poorly understood. Here we demonstrate for the first time that a Cut-like homeodomain transcription factor family member, Cux2 (Cutl2), regulates cell-cycle progression and development of neural progenitors. Cux2 ... More
SHAP potentiates the CD44-mediated leukocyte adhesion to the hyaluronan substratum.
AuthorsZhuo L; Kanamori A; Kannagi R; Itano N; Wu J; Hamaguchi M; Ishiguro N; Kimata K
JournalThe Journal of Biological Chemistry
PubMed ID
CD44-hyaluronan (HA) interaction is involved in diverse physiological and pathological processes. Regulation of interacting avidity is well studied on CD44 but rarely on HA. We discovered a unique covalent modification of HA with a protein, SHAP, that corresponds to the heavy chains of inter-alpha-trypsin inhibitor family molecules circulating in blood. ... More
Cbl-dependent ubiquitination is required for progression of EGF receptors into clathrin-coated pits.
AuthorsStang E; Blystad FD; Kazazic M; Bertelsen V; Brodahl T; Raiborg C; Stenmark H; Madshus IH
JournalMolecular Biology of the Cell
PubMed ID
Ligand binding causes the EGF receptor (EGFR) to become ubiquitinated by Cbl upon association with the adaptor protein Grb2. We have investigated the role of ubiquitin and Grb2 in ligand-induced endocytosis of the EGFR. Incubation of cells with EGF on ice caused translocation of Grb2 and Cbl from the cytosol ... More
Characterization of the Type III restriction endonuclease PstII from Providencia stuartii.
AuthorsSears A; Peakman LJ; Wilson GG; Szczelkun MD
JournalNucleic Acids Research
PubMed ID
A new Type III restriction endonuclease designated PstII has been purified from Providencia stuartii. PstII recognizes the hexanucleotide sequence 5'-CTGATG(N)(25-26/27-28)-3'. Endonuclease activity requires a substrate with two copies of the recognition site in head-to-head repeat and is dependent on a low level of ATP hydrolysis ( approximately 40 ATP/site/min). Cleavage ... More
Characterization of Endophilin B1b, a brain-specific membrane-associated lysophosphatidic acid acyl transferase with properties distinct from endophilin A1.
AuthorsModregger J; Schmidt AA; Ritter B; Huttner WB; Plomann M
JournalThe Journal of Biological Chemistry
PubMed ID
We have characterized mammalian endophilin B1, a novel member of the endophilins and a representative of their B subgroup. The endophilins B show the same domain organization as the endophilins A, which contain an N-terminal domain responsible for lipid binding and lysophosphatidic acid acyl transferase activity, a central coiled-coil domain ... More
A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro.
AuthorsWang Y; Prosen DE; Mei L; Sullivan JC; Finney M; Vander Horn PB
JournalNucleic Acids Research
PubMed ID
Mechanisms that allow replicative DNA polymerases to attain high processivity are often specific to a given polymerase and cannot be generalized to others. Here we report a protein engineering-based approach to significantly improve the processivity of DNA polymerases by covalently linking the polymerase domain to a sequence non-specific dsDNA binding ... More
Differentiation of embryonic stem cells conditionally expressing neurogenin 3.
Expression of the proendocrine gene neurogenin 3 (Ngn3) is required for the development of pancreatic islets. To better characterize the molecular events regulated by Ngn3 during development, we have determined the expression profiles of murine embryonic stem cells (mESCs) uniformly induced to overexpress Ngn3. An mESC line was created in ... More
T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody.
AuthorsWhite RA, Mason DW, Williams AF, Galfre G, Milstein C,
JournalJ Exp Med
PubMed ID29936
'W3/25 antibody is the monoclonal product of a hybrid cell resulting from the fusion of a mouse myeloma cell line with spleen cells from a mouse immunized with rat thymocytes. Pure clones have been derived, and segregants free of parental myeloma chains have been isolated. Previous studies have shown that ... More
The hepatitis B virus PRE contains a splicing regulatory element.
AuthorsHeise T; Sommer G; Reumann K; Meyer I; Will H; Schaal H
Journal
PubMed ID
'The posttranscriptional regulatory element (PRE) is considered to enhance hepatitis B virus (HBV) gene expression by facilitating the nuclear export of intronless viral subgenomic RNAs. Its role in the RNA metabolism of the viral pregenomic RNA (pgRNA) is currently unknown. We identified a positively cis-acting splicing regulatory element (SRE-1) and ... More
Detection and identification of human papillomavirus in cervical adenocarcinoma
AuthorsGuo, Y; Li, W; Wang, Q; Li, H
Journal
PubMed ID
'Objective: To evaluate the rate and types of human papillomavirus (HPV) infection in cervical adenocarcinoma. Methods: We detected and identified HPV in 67 lesions using PCR based reverse line blot hybridization and DNA sequencing. Among the 67 patients, 53 were diagnosed as cervical adenocarcinoma and 14 as cervical adenosquamous carcinoma. ... More
Intratracheal adenoviral-mediated delivery of iNOS decreases pulmonary vasoconstrictor responses in rats.
'We hypothesized that adenovirus-mediated inducible nitric oxide synthase (iNOS) gene transduction of the lung would result in time-dependent iNOS overexpression and attenuate the vascular constrictor responses to a thromboxane mimetic, U-46619. Rats were treated via the trachea with surfactant alone (sham), surfactant containing an adenoviral construct with a cytomegalovirus promoter-regulated ... More
Detection of diverse new Francisella-like bacteria in environmental samples.
'Following detection of putative Francisella species in aerosol samples from Houston, Texas, we surveyed soil and water samples from the area for the agent of tularemia, Francisella tularensis, and related species. The initial survey used 16S rRNA gene primers to detect Francisella species and related organisms by PCR amplification of ... More
Eliminating the synthesis of mature lamin A reduces disease phenotypes in mice carrying a Hutchinson-Gilford progeria syndrome allele
'Hutchinson-Gilford progeria syndrome is caused by the synthesis of a mutant form of prelamin A, which is generally called progerin. Progerin is targeted to the nuclear rim, where it interferes with the integrity of the nuclear lamina, causes misshapen cell nuclei, and leads to multiple aging-like disease phenotypes. We created ... More
Transmission of iridovirus from freshwater ornamental fish (pearl gourami) to marine fish (rock bream)
AuthorsJeong, JB; Cho, HJ; Jun, LJ; Hong, SH; Chung, JK; Do Jeong, H
Journal
PubMed ID
'Freshwater pearl gourami Trichogaster leeri and seawater rock bream Oplegnathus fasciatus infected by the iridoviruses PGIV-SP and IVS-1 were carrying similar numbers of viral particles (2.52 x 10(8) and 2.46 x 10(8) viral genome copies mg(-1) spleen tissue, respectively). The viral genome copy number for both iridoviruses decreased much faster ... More
Mouse paneth cell secretory responses to cell surface glycolipids of virulent and attenuated pathogenic bacteria.
AuthorsTanabe H; Ayabe T; Bainbridge B; Guina T; Ernst RK; Darveau RP; Miller SI; Ouellette AJ
Journal
PubMed ID
'Mouse Paneth cells respond to bacteria and bacterial cell surface antigens by discharging secretory granules into the lumen of small intestinal crypts (T. Ayabe et al., Nat. Immunol. 1:113-118, 2000). To investigate mechanisms regulating these responses, purified surface glycolipid molecules with known acyl chain modifications and attenuated properties were tested ... More
Global transcriptional response of Bacillus subtilis to treatment with subinhibitory concentrations of antibiotics that inhibit protein synthesis.
'Global gene expression patterns of Bacillus subtilis in response to subinhibitory concentrations of protein synthesis inhibitors (chloramphenicol, erythromycin, and gentamicin) were studied by DNA microarray analysis. B. subtilis cultures were treated with subinhibitory concentrations of protein synthesis inhibitors for 5, 15, 30, and 60 min, and transcriptional patterns were measured ... More
The biofilm life cycle and virulence of Pseudomonas aeruginosa are dependent on a filamentous prophage
AuthorsRice, SA; Tan, CH; Mikkelsen, PJ; Kung, V; Woo, J; Tay, M; Hauser, A; McDougald, D; Webb, JS; Kjelleberg, S
Journal
PubMed ID
'Mature Pseudomonas aeruginosa biofilms undergo specific developmental events. Using a bacteriophage mutant, generated by deletion of the entire filamentous Pf4 prophage, we show that the phage is essential for several stages of the biofilm life cycle and that it significantly contributes to the virulence of P. aeruginosa in vivo. Here, ... More
Multilocus sequence typing system for Campylobacter jejuni.
AuthorsDingle KE; Colles FM; Wareing DR; Ure R; Fox AJ; Bolton FE; Bootsma HJ; Willems RJ; Urwin R; Maiden MC
Journal
PubMed ID
'The gram-negative bacterium Campylobacter jejuni has extensive reservoirs in livestock and the environment and is a frequent cause of gastroenteritis in humans. To date, the lack of (i) methods suitable for population genetic analysis and (ii) a universally accepted nomenclature has hindered studies of the epidemiology and population biology of ... More
Identification and Localization of Myosin Superfamily Members in Fish Retina and Retinal Pigmented Epithelium
'Myosins are cytoskeletal motors critical for generating the forces necessary for establishing cell structure and mediating actin-dependent cell motility. In each cell type a multitude of myosins are expressed, each myosin contributing to aspects of morphogenesis, transport, or motility occurring in that cell type. To examine the roles of myosins ... More
Characterization of a recurrent germ line mutation of the E-cadherin gene: implications for genetic testing and clinical management.
AuthorsSuriano G; Yew S; Ferreira P; Senz J; Kaurah P; Ford JM; Longacre TA; Norton JA; Chun N; Young S; Oliveira MJ; Macgillivray B; Rao A; Sears D; Jackson CE; Boyd J; Yee C; Deters C; Pai GS; Hammond LS; McGivern BJ; Medgyesy D; Sartz D; Arun B; Oelschlager B
Journal
PubMed ID
'PURPOSE: To identify germ line CDH1 mutations in hereditary diffuse gastric cancer (HDGC) families and develop guidelines for management of at risk individuals. EXPERIMENTAL DESIGN: We ascertained 31 HDGC previously unreported families, including 10 isolated early-onset diffuse gastric cancer (DGC) cases. Screening for CDH1 germ line mutations was done by ... More
Tandemly duplicated acyl carrier proteins, which increase polyketide antibiotic production, can apparently function either in parallel or in series.
AuthorsRahman AS; Hothersall J; Crosby J; Simpson TJ; Thomas CM
Journal
PubMed ID
'Polyketide biosynthesis involves the addition of subunits commonly derived from malonate or methylmalonate to a starter unit such as acetate. Type I polyketide synthases are multifunctional polypeptides that contain one or more modules, each of which normally contains all the enzymatic domains for a single round of extension and modification ... More
MicroRNAs (miR)-221 and miR-222, both overexpressed in human thyroid papillary carcinomas, regulate p27Kip1 protein levels and cell cycle.
'We have recently reported that MicroRNAs (miR)-221 and miR-222 were up-regulated in human thyroid papillary carcinomas in comparison with the normal thyroid tissue. Bioinformatic analysis proposed the p27(Kip1) protein, a key regulator of cell cycle, as a candidate target for the miR-221/222 cluster. Here, we report that the enforced expression ... More
Role of a conserved tripeptide in the endodomain of Sindbis virus glycoprotein E2 in virus assembly and function.
AuthorsWest J; Brown DT
Journal
PubMed ID
'Envelopment of Sindbis virus (SV) at the plasma membrane begins with the interaction of the E2 glycoprotein endodomain with a hydrophobic cleft in the surface of the pre-assembled nucleocapsid. The driving force for this budding event is thought to reside in this virus type-specific association at the surface of the ... More
Porcine Parkin: molecular cloning of PARK2 cDNA, expression analysis, and identification of a splicing variant.
AuthorsBjerre D; Madsen LB; Bendixen C; Larsen K
Journal
PubMed ID
'Parkin, encoded by the PARK2 gene, is an E3 ligase which functions as an integral component of the cytoplasmic ubiquitin/proteasomal protein degradation pathway. Mutations in the PARK2 gene, resulting in the loss of parkin function, leads to autosomal recessive juvenile Parkinsonism (AR-JP). This work reports the cloning and characterization of ... More
Global Hypomethylation of Genomic DNA in Cancer-Associated Myofibroblasts
AuthorsJiang, L; Gonda, TA; Gamble, MV; Salas, M; Seshan, V; Tu, S; Twaddell, WS; Hegyi, P; Lazar, G; Steele, I; Varro, A; Wang, TC; Tycko, B
Journal
PubMed ID
'Global hypomethylation has long been recognized as a feature of the malignant epithelial component in human carcinomas. Here we show evidence for this same type of epigenetic alteration in cancer-associated stromal myofibroblasts. We used methylation-sensitive SNP array analysis (MSNP) to profile DNA methylation in early-passage cultures of stromal myofibroblasts isolated ... More
OvcaI, a candidate gene of the genetic modifier of Tp53, Mop2, affects mouse embryonic lethality
Authors
Journal
PubMed ID
'In this study, we show genetic modifier genes of Tp53 that can exacerbate embryonic abnormalities. Using a mouse model in which CE/J mice were crossed with the Tp53-null 129/Sv (129-Trp53(tml) (Tyj)) mice, a subset of Tp53+/- and -/- male and female embryos died during gestation. Our hypothesis, based on the ... More
Extended core 1 and core 2 branched O-glycans differentially modulate sialyl Lewis X-type L-selectin ligand activity.
'It has been established that sialyl Lewis x in core 2 branched O-glycans serves as an E- and P-selectin ligand. Recently, it was discovered that 6-sulfosialyl Lewis x in extended core 1 O-glycans, NeuNAcalpha2-->3Galbeta1-->4(Fucalpha1-->3(sulfo-->6))GlcNAcbeta1--> 3Galbeta1-->3GalNAcalpha1-->Ser/Thr, functions as an L-selectin ligand in high endothelial venules. Extended core 1 O-glycans can be ... More
Population Structure of the Lyme Borreliosis Spirochete Borrelia burgdorferi in the Western Black-Legged Tick (Ixodes pacificus) in Northern California
'Factors potentially contributing to the lower incidence of Lyme borreliosis (LB) in the far-western than in the northeastern United States include tick host-seeking behavior resulting in fewer human tick encounters, lower densities of Borrelia burgdorferi-infected vector ticks in peridomestic environments, and genetic variation among B. burgdorferi spirochetes to which humans ... More
A multi-locus phylogeny for Phytophthora utilizing markers derived from complete genome sequences
AuthorsBlair, JE; Coffey, MD; Park, SY; Geiser, DM; Kang, S
Journal
PubMed ID
'Phytophthora species are devastating plant pathogens in both agricultural and natural environments. Due to their significant economic and environmental impact, there has been increasing interest in Phytophthora genetics and genomics, culminating in the recent release of three complete genome sequences (P. ramorum, P. sojae, and P. infestans). In this study, ... More
Use of variable-number tandem repeats to examine genetic diversity of Neisseria meningitidis.
AuthorsYazdankhah SP; Lindstedt BA; Caugant DA
Journal
PubMed ID
'Repetitive DNA motifs with potential variable-number tandem repeats (VNTR) were identified in the genome of Neisseria meningitidis and used to develop a typing method. A total of 146 meningococcal isolates recovered from carriers and patients were studied. These included 82 of the 107 N. meningitidis isolates previously used in the ... More
Fine mapping and association analysis of a quantitative trait locus for milk production traits on Bos taurus autosome 4
'To fine map a quantitative trait locus (QTL) affecting milk production traits previously associated with microsatellite RM188, we implemented an interval mapping analysis by using microsatellite markers in a large Israeli Holstein half-sib sire family, and linkage disequilibrium (LD) mapping in a large set of US Holstein bulls. Interval mapping ... More
Aortic valve endocarditis in a dog due to Bartonella clarridgeiae.
AuthorsChomel BB; Mac Donald KA; Kasten RW; Chang CC; Wey AC; Foley JE; Thomas WP; Kittleson MD
Journal
PubMed ID
'We report the first documented case of endocarditis associated with Bartonella clarridgeiae in any species. B. clarridgeiae was identified as a possible etiological agent of human cat scratch disease. Infective vegetative valvular aortic endocarditis was diagnosed in a 2.5-year-old male neutered boxer. Historically, the dog had been diagnosed with a ... More
Uteroplacental insufficiency affects epigenetic determinants of chromatin structure in brains of neonatal and juvenile IUGR rats.
AuthorsKe X; Lei Q; James SJ; Kelleher SL; Melnyk S; Jernigan S; Yu X; Wang L; Callaway CW; Gill G; Chan GM; Albertine KH; McKnight RA; Lane RH
Journal
PubMed ID
'Intrauterine growth retardation (IUGR) increases the risk of neuroendocrine reprogramming. In the rat, IUGR leads to persistent changes in cerebral mRNA levels. This suggests lasting alterations in IUGR cerebral transcriptional regulation, which may result from changes in chromatin structure. Candidate nutritional triggers for these changes include altered cerebral zinc and ... More
Phylogenetic relationships of the Aurantioideae (Rutaceae) based on the nuclear ribosomal DNA ITS region and three noncoding chloroplast DNA regions, atpB-rbcL spacer, rps16, and trnL-trnF
AuthorsMorton, CM
Journal
PubMed ID
'The tribes and subtribes of Aurantioideae, an economically important subfamily of the Rutaceae, have a controversial taxonomic history because a phylogenetic framework has been lacking. In order to construct an evolutionary history and evaluate the most recent classification system [Swingle and Reece 1967. The botany of Citrus and its wild ... More
Lack of correlation between phenotype and genotype in untreated 21-hydroxylase-deficient Indonesian patients
'Background Mutations in CYP21A2 lead to deficiency of 21-hydroxylase and can have either severe or moderate effects on phenotype, which can be prevented by early treatment. We studied long-term effects of this deficiency on phenotype in patients who had not been treated for prolonged periods and correlated these phenotypes ... More
Genetic characterisation of the ethambutol resistance-determining region in Mycobacterium tuberculosis: prevalence and significance of embB306 mutations
AuthorsPerdigao, J; Macedo, R; Ribeiro, A; Brum, L; Portugal, I
Journal
PubMed ID
'Ethambutol (EMB) is a first-line antitubercular drug that inhibits arabinogalactan and lipoarabinomannan biosynthesis. Resistance to EMB has traditionally been associated with embB mutations, especially in the Met306 codon. In this study, the region encompassing the embB306 codon in 109 Mycobacterium tuberculosis clinical isolates (49 EMB-susceptible and 60 EMB-resistant) was characterised. ... More
Diminished antigen-specific IgG1 and interleukin-6 production and acetylcholinesterase expression in combined M1 and M5 muscarinic acetylcholine receptor knockout mice.
AuthorsFujii YX; Tashiro A; Arimoto K; Fujigaya H; Moriwaki Y; Misawa H; Fujii T; Matsui M; Kasahara T; Kawashima K
Journal
PubMed ID
'Immunological activation of T cells enhances synthesis of acetylcholine (ACh) and transcription of choline acetyltransferase (ChAT), M5 muscarinic ACh receptor (mAChR) and acetylcholinesterase (AChE). Stimulation of mAChRs on T and B cells causes oscillating Ca(2+)-signaling and up-regulation of c-fos expression; moreover, M1 mAChRs play a crucial role in the differentiation ... More
Human Papillomavirus Type 16 (HPV-16) Virus-Like Particle L1-Specific CD8(+) Cytotoxic T Lymphocytes (CTLs) Are Equally Effective as E7-Specific CD8(+) CTLs in Killing Autologous HPV-16-Positive Tumor Cells in Cervical Cancer Patients: Implications f
AuthorsBellone, S; El-Sahwi, K; Cocco, E; Casagrande, F; Cargnelutti, M; Palmieri, M; Bignotti, E; Romani, C; Silasi, DA; Azodi, M; Schwartz, PE; Rutherford, TJ; Pecorelli, S; Santin, AD
Journal
PubMed ID
'Papillomavirus-like particles (VLPs) based on L1 capsid protein represent a promising prophylactic vaccine against human papillomavirus (HPV) infections. However, cell-mediated immune responses against this antigen are believed to be of limited therapeutic value in established HPV-infected cervical lesions and, for this reason, have not been intensively investigated in cervical cancer ... More
The CpG island methylator phenotype and chromosomal instability are inversely correlated in sporadic colorectal cancer.
'BACKGROUND & AIMS: The CpG island methylator phenotype (CIMP) is one of the mechanisms involved in colorectal carcinogenesis (CRC). Although CIMP is probably the cause of high-frequency microsatellite instability (MSI-H) sporadic CRCs, its role in microsatellite stable (MSS) tumors is debated. The majority of MSS CRCs demonstrate chromosomal instability (CIN) ... More
Retrovirus-mediated in vitro gene transfer into chicken male germ line cells.
AuthorsKalina J; Senigl F; Micakova A; Mucksova J; Blazkova J; Yan H; Poplstein M; Hejnar J; Trefil P
Journal
PubMed ID
'Chicken testicular cells, including spermatogonia, transplanted into the testes of recipient cockerels sterilized by repeated gamma-irradiation repopulate the seminiferous epithelium and resume the exogenous spermatogenesis. This procedure could be used to introduce genetic modifications into the male germ line and generate transgenic chickens. In this study, we present a successful ... More
A novel mutation (T61I) in the gene encoding tumour necrosis factor receptor superfamily 1A (TNFRSF1A) in a Japanese patient with tumour necrosis factor receptor-associated periodic syndrome (TRAPS) associated with systemic lupus erythematosus.
AuthorsIda H; Kawasaki E; Miyashita T; Tanaka F; Kamachi M; Izumi Y; Huang M; Tamai M; Origuchi T; Kawakami A; Migita K; Motomura M; Yoshimura T; Eguchi K
Journal
PubMed ID
'OBJECTIVE: To identify potential mutations in the tumour necrosis factor receptor superfamily 1A gene (TNFRSF1A) in a Japanese female patient with recurrent fever complicated by systemic lupus erythematosus (SLE), and in her family members. METHODS: DNA sequencing of exons 1-10 of the TNFRSF1A gene was performed to determine mutations that ... More
Differential expression of placental villous angiopoietin-1 and -2 during early, mid and late baboon pregnancy.
AuthorsBabischkin JS; Suresch DL; Pepe GJ; Albrecht ED
Journal
PubMed ID
'Although vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1) and Ang-2 have important roles in angiogenesis, very little is known about the regulation of these factors in the villous placenta during human pregnancy. In the present study, to investigate whether placental expression of Ang-1, Ang-2 and VEGF was altered in a ... More
Identification to the species level and differentiation between strains of Aspergillus clinical isolates by automated repetitive-sequence-based PCR.
AuthorsHealy M; Reece K; Walton D; Huong J; Shah K; Kontoyiannis DP
Journal
PubMed ID
'A commercially available repetitive-sequence-based PCR (rep-PCR) DNA fingerprinting assay adapted to an automated format, the DiversiLab system, enables rapid microbial identification and strain typing. We explored the performance of the DiversiLab system as a molecular typing tool for 69 Aspergillus isolates (38 A. fumigatus, 15 A. flavus, and 16 A. ... More
Preimplantation genetic diagnosis for BRCA1 exon 13 duplication mutation using linked polymorphic markers resulting in a live birth
AuthorsJasper, MJ; Liebelt, J; Hussey, ND
Journal
PubMed ID
'Background The risk of breast cancer associated with inheriting a BRCA1 mutation is extremely high, in addition, there is a 50% chance of transmitting this familial cancer mutation to any offspring. Methods A 31-year-old woman with a strong maternal family history of early onset of breast cancer had experienced ... More
Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR
'The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and ... More
TNF-α and IL-10 gene polymorphisms versus cardioimmunological responses in sudden infant death
'We hypothesized that genetic variations of cytokines could contribute to the risk of developing a fatal immunological reaction in the heart of infants. Thus, tumor necrosis factor (TNF)-α and interleukin (IL)-10 gene polymorphisms versus induction of cardioimmunologxical responses in victims of sudden infant death syndrome (SIDS) were explored. We genotyped ... More
Neospora caninum: high susceptibility to the parasite in C57BL/10ScCr mice.
'C57BL/10ScCr mice, lack Toll-like receptor 4 and a functional Interleukin-12 receptor. Taking this into account, susceptibility of these mice to Neospora caninum infection was assessed comparatively to that of immunocompetent C57BL/10ScSn mice. C57BL/10ScCr mice inoculated intraperitoneally with 5x10(5)N. caninum tachyzoites showed a high susceptibility to this parasite. All infected C57BL/10ScCr ... More
Ligase detection reaction for the analysis of point mutations using free-solution conjugate electrophoresis in a polymer microfluidic device
'We have developed a new method for the analysis of low abundant point mutations in genomic DNA using a combination of an allele-specific ligase detection reaction (LDR) with free-solution conjugate electrophoresis (FSCE) to generate and analyze the genetic products. FSCE eliminates the need for a polymer sieving matrix by conjugating ... More
Molecular identification of zygomycetes from culture and experimentally infected tissues.
'Mucormycosis is an emerging infection associated with a high mortality rate. Identification of the causative agents remains difficult and time-consuming by standard mycological procedures. In this study, internal transcribed spacer (ITS) sequencing was validated as a reliable technique for identification of Zygomycetes to the species level. Furthermore, species identification directly ... More
Increased survival, proliferation, and migration in metastatic human pancreatic tumor cells expressing functional CXCR4.
'In this study, we have evaluated 11 pancreatic tumor cell lines and tumor cells from surgical samples of patients with pancreatic adenocarcinoma for expression of the chemokine receptor CXCR4. Six of 11 cell lines expressed detectable mRNA of CXCR4, with three cell lines (AsPC1, Capan1, and Hs766T) having substantial amounts ... More
Transcriptional gene silencing promotes DNA hypermethylation through a sequential change in chromatin modifications in cancer cells.
AuthorsStirzaker C; Song JZ; Davidson B; Clark SJ
Journal
PubMed ID
'It is well established that DNA hypermethylation of tumor suppressor and tumor-related genes can occur in cancer cells and that each cancer subtype has specific gene sets that are commonly susceptible to methylation and silencing. Glutathione S-transferase (GSTP1) is one example of a gene that is hypermethylated and inactivated in ... More
Comparative genomics of Neisseria meningitidis: core genome, islands of horizontal transfer and pathogen-specific genes.
AuthorsHotopp JC; Grifantini R; Kumar N; Tzeng YL; Fouts D; Frigimelica E; Draghi M; Giuliani MM; Rappuoli R; Stephens DS; Grandi G; Tettelin H
Journal
PubMed ID
'To better understand Neisseria meningitidis genomes and virulence, microarray comparative genome hybridization (mCGH) data were collected from one Neisseria cinerea, two Neisseria lactamica, two Neisseria gonorrhoeae and 48 Neisseria meningitidis isolates. For N. meningitidis, these isolates are from diverse clonal complexes, invasive and carriage strains, and all major serogroups. The ... More
Geographical variation in serological responses to recombinant Pneumocystis jirovecii major surface glycoprotein antigens
AuthorsDaly, K; Koch, J; Respaldiza, N; de la Horra, C; Montes-Cano, MA; Medrano, FJ; Varela, JM; Calderon, EJ; Walzer, PD
Journal
PubMed ID
'The use of recombinant fragments of the major surface glycoprotein (Msg) of Pneumocystis jirovecii has proven useful for studying serological immune responses of blood donors and human immunodeficiency virus (HIV)-positive (HIV+) patients. Here, we have used ELISA to measure antibody titres to Msg fragments (MsgA, MsgB, MsgC1, MsgC3, MsgC8 and ... More
Variations in Prkdc and susceptibility to benzene-induced toxicity in mice.
AuthorsFaiola B; Bauer AK; Fuller ES; Wong VA; Pluta LJ; Abernethy DJ; Mangum JB; Everitt JI; Recio L
Journal
PubMed ID
'Benzene, a carcinogen that induces chromosomal breaks, is strongly associated with leukemias in humans. Possible genetic determinants of benzene susceptibility include proteins involved in repair of benzene-induced DNA damage. The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), encoded by Prkdc, is one such protein. DNA-PKcs is involved in the nonhomologous ... More
Cloning, expression, and site-directed mutagenesis of the propene monooxygenase genes from Mycobacterium sp. strain M156.
AuthorsChan Kwo Chion CK; Askew SE; Leak DJ
Journal
PubMed ID
'Propene monooxygenase has been cloned from Mycobacterium sp. strain M156, based on hybridization with the amoABCD genes of Rhodococcus corallinus B276. Sequencing indicated that the mycobacterial enzyme is a member of the binuclear nonheme iron monooxygenase family and, in gene order and sequence, is most similar to that from R. ... More
Regulation of placental villous angiopoietin-1 and -2 expression by estrogen during baboon pregnancy
AuthorsAlbrecht, ED; Babischkin, JS; Pepe, GJ
Journal
PubMed ID
'We recently showed an increase in vascular endothelial growth factor (VEGF), decrease in angiopoietin-1 (Ang-1) and unaltered Ang-2 expression by the villous placenta with advancing baboon pregnancy. Moreover, placental VEGF expression was increased by estrogen in early pregnancy. In the present study, we determined whether placental Ang-1 and Ang-2 are ... More
Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells.
'Mouse embryonic stem (ES) cells were used as an experimental model to study the effects of electromagnetic fields (EMF). ES-derived nestin-positive neural progenitor cells were exposed to extremely low frequency EMF simulating power line magnetic fields at 50 Hz (ELF-EMF) and to radiofrequency EMF simulating the Global System for Mobile ... More
Regionally high rates of hybridization and introgression in German wildcat populations (Felis silvestris, Carnivora, Felidae)
AuthorsHertwig, ST; Schweizer, M; Stepanow, S; Jungnickel, A; Boehle, UR; Fischer, MS
Journal
PubMed ID
'While the western populations of the wildcat (Felis silvestris silvestris) in Germany come into contact with wildcats in France and Switzerland, the eastern distribution area is geographically completely isolated and consists of scattered subpopulations. To investigate population structure, evolutionary relationships and degree of hybridization with domestic cats we analysed the ... More
Killer immunoglobulin-like receptor repertoire on uterine natural killer cell subsets in women with recurrent spontaneous abortions
'Objective: The objective of this study is to investigate the distribution of inhibitory and activating killer immunoglobulin-like receptors (KIRs) on uterine natural killer (uNK) cells and the compatibility of KIR/HLA-C at the maternal-fetal interface in women with unexplained recurrent spontaneous abortion (RSA) in the Chinese population. Study design: Sixteen patients ... More
Sequential reductive dechlorination of meta-chlorinated polychlorinated biphenyl congeners in sediment microcosms by two different Chloroflexi phylotypes.
AuthorsFagervold SK; Watts JE; May HD; Sowers KR
Journal
PubMed ID
'Three species within a deeply branching cluster of the Chloroflexi are the only microorganisms currently known to anaerobically transform polychlorinated biphenyls (PCBs) by the mechanism of reductive dechlorination. A selective PCR primer set was designed that amplifies the 16S rRNA genes of a monophyletic group within the Chloroflexi including Dehalococcoides ... More
Expression and actions of transforming growth factors during human follicular development
AuthorsLobb, DK
Journal
PubMed ID
'Objective: To correlate the temporal expression of transforming growth factor-α (TGF-α) and TGF-β genes in granulosa cells (GC) and thecal cells from human follicles at various developmental stages and to determine their trophic effects on GCs. Design: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of extracted RNA from follicular GCs ... More
Mutations associated with failure of raltegravir treatment affect integrase sensitivity to the inhibitor in vitro
'Raltegravir (MK-0518) is a potent inhibitor of human immunodeficiency virus (HIV) integrase and is clinically effective against viruses resistant to other classes of antiretroviral agents. However, it can select mutations in the HIV integrase gene. Nine heavily pretreated patients who received salvage therapy including raltegravir and who subsequently developed virological ... More
Molecular characterization of ciliate diversity in stream biofilms.
AuthorsDopheide A; Lear G; Stott R; Lewis G
Journal
PubMed ID
'Free-living protozoa are thought to be of fundamental importance in aquatic ecosystems, but there is limited understanding of their diversity and ecological role, particularly in surface-associated communities such as biofilms. Existing eukaryote-specific PCR primers were used to survey 18S rRNA gene sequence diversity in stream biofilms but poorly revealed protozoan ... More
Molecular and functional characterization of O antigen transfer in Vibrio cholerae.
AuthorsSchild S; Lamprecht AK; Reidl J
Journal
PubMed ID
'The majority of Gram-negative bacteria transfer O antigen polysaccharides onto the lipid A-core oligosaccharide via the action of surface polymer:lipid A-core ligases (WaaL). Here, we characterize the WaaL proteins of Vibrio cholerae with emphasis on structural and functional characterization of O antigen transfer and core oligosaccharide recognition. We demonstrate that ... More
Genetic relatedness and virulence gene profiles of Escherichia coli strains isolated from septicaemic and uroseptic patients
'We investigated the relationship between clonality and virulence factors (VFs) of a collection of Escherichia coli strains isolated from septicaemic and uroseptic patients with respect to their origin of translocation. Forty septicaemic and 30 uroseptic strains of E. coli were tested for their phylogenetic groupings, genetic relatedness using randomly amplified ... More
Impact of adiponectin gene polymorphisms on plasma lipoprotein and adiponectin concentrations of viscerally obese men.
AuthorsBerthier MT; Houde A; Cote M; Paradis AM; Mauriege P; Bergeron J; Gaudet D; Despres JP; Vohl MC
Journal
PubMed ID
'The aim of this study was first to examine the relationships between adiponectin gene (Apm1) polymorphisms and anthropometric indices as well as plasma adiponectin and lipoprotein/lipid levels, and then to investigate whether the presence of visceral obesity or insulin resistance may modulate the impact of these polymorphisms on metabolic risk ... More
Conversion of cell movement responses to Semaphorin-1 and Plexin-1 from attraction to repulsion by lowered levels of specific RAC GTPases in C. elegans.
AuthorsDalpe G; Zhang LW; Zheng H; Culotti JG
Journal
PubMed ID
'Plexins are functional receptors for Semaphorin axon guidance cues. Previous studies have established that some Plexins directly bind RAC(GTP) and RHO. Recent work in C. elegans showed that semaphorin 1 (smp-1 and smp-2) and plexin 1 (plx-1) are required to prevent anterior displacement of the ray 1 cells in the ... More
Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information
'DNA markers able to distinguish species or genera with high specificity are valuable in the identification of introgressed regions in interspecific or intergeneric hybrids. Intergeneric hybridization between the genera of Lolium and Festuca, leading to the reciprocal introgression of chromosomal segments, can produce novel forage grasses with unique combinations of ... More
Characterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96.
AuthorsChoi YJ; Miguez CB; Lee BH
Journal
PubMed ID
'A novel esterase gene (estI) of Lactobacillus casei CL96 was localized on a 3.3-kb BamHI DNA fragment containing an open reading frame (ORF) of 1,800 bp. The ORF of estI was isolated by PCR and expressed in Escherichia coli, the methylotrophic bacterium Methylobacterium extorquens, and the methylotrophic yeast Pichia pastoris ... More