AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

AmpliTaq DNA-Polymerase ist eine 94 kDa große, thermostabile, rekombinante DNA-Polymerase, die durch Expression einer modifizierten Form des Thermus aquaticus (Taq)-DNA-Polymerase-GensWeitere Informationen
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KatalognummerEnthältMenge
N8080160Buffer I250 Einheiten
N8080161Buffer II250 Einheiten
N8080172Buffer II1.000 Einheiten
N8080153Buffer II3000 Einheiten
N8080156Buffer II5000 Einheiten
N8080186Buffer II25.000 Einheiten
N8080152Buffer I3000 Einheiten
N8080155Buffer I5000 Einheiten
N8080171Buffer I1000 Einheiten
N8080185Buffer I25000 Einheiten
Katalognummer N8080160
Preis (EUR)
270,00
Each
Zum Warenkorb hinzufügen
Enthält:
Buffer I
Menge:
250 Einheiten
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
270,00
Each
Zum Warenkorb hinzufügen
AmpliTaq DNA-Polymerase ist eine 94 kDa große, thermostabile, rekombinante DNA-Polymerase, die durch Expression einer modifizierten Form des Thermus aquaticus (Taq)-DNA-Polymerase-Gens in E. coli gewonnen wird. Eines der spezifischsten für die PCR verfügbaren Enzyme, dessen rekombinante Eigenschaften sowie seine Reinigungsmethode für unvergleichliche, fläschchen- sowie chargenübergreifende Reinheit und Reproduzierbarkeit sorgen.

Merkmale dieses Enzyms:

  • Die AmpliTaq DNA-Polymerase ist eines der am besten beschriebenen, für die PCR verfügbaren Enzyme, was ihren allgemeine Nutzen und ihre Effektivität bezeugt
  • Dank thermischem Aktivitätsprofil eine optimale Wahl für PCR-Anwendungen
  • QC-getestet, um reproduzierbare Ergebnisse zu gewährleisten

  • Zuverlässige und robuste PCR
    Das thermische Aktivitätsprofil von AmpliTaq DNA-Polymerase eignet sich gut für PCR-Anwendungen, da seine optimale Aktivität in demselben Bereich liegt, in dem das Annealing von Primern auftritt (55 – 75 °C). Die Halbwertszeit des Enzyms beträgt ∼40 Minuten bei 95 °C und bietet so eine Thermostabilität, die den Anforderungen der schwierigsten PCR-Anwendungen entspricht. Diese AmpliTaq DNA-Polymerase wird mit GeneAmp 10X PCR-Puffer II und MgCl2-Lösung geliefert.

    Für eine hervorragende PCR-Leistung empfehlen wir die DreamTaq DNA-Polymerase.

    Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

    Specifications
    Exonukleaseaktivität5' – 3'
    Wiedergabetreue (vs. Taq)1X
    FormatEigenständiges Enzym
    Hot-StartNein
    EnthältBuffer I
    Anzahl Reaktionen200 Reaktionen
    Überhang3'-A
    PolymeraseAmpliTaq DNA-Polymerase
    ProdukttypDNA-Polymerase
    Menge250 Einheiten
    ReaktionsformatSeparate Komponenten
    VersandbedingungTrockeneis
    Größe (Endprodukt)5 kb oder weniger
    AusgangsmaterialDNA
    Konzentration5 E/μl, 10X
    Zur Verwendung mit (Anwendung)Standard-PCR
    ReaktionsgeschwindigkeitStandard
    Unit SizeEach
    Inhalt und Lagerung
    Inhalt:
    • 50 μl AmpliTaq™ DNA-Polymerase (5 E/μl)
    • 1,5 ml GeneAmp™ 10X-PCR-Puffer I (100 mM Tris-HCl, pH 8,3, 500 mM KCl, 15 mM MgCl2, 0,01 % (w/v) Gelatine).
    Lagerung bei -20 °C.

    Häufig gestellte Fragen (FAQ)

    My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

    Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

    The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

    Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

    I don't see a pellet in my oligo tube order. Should I ask for a replacement?

    The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

    There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

    If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

    There is a green color in my lyophilized oligo. Can I still use it?

    If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

    Zitierungen und Referenzen (1798)

    Zitierungen und Referenzen
    Abstract
    Chimpanzee Fab fragments and a derived humanized immunoglobulin G1 antibody that efficiently cross-neutralize dengue type 1 and type 2 viruses.
    Authors:Goncalvez AP; Men R; Wernly C; Purcell RH; Lai CJ
    Journal:Journal of Virology
    PubMed ID:
    Passive immunization with monoclonal antibodies from humans or nonhuman primates represents an attractive alternative to vaccines for prevention of illness caused by dengue viruses (DENV) and other flaviviruses, including the West Nile virus. In a previous study, repertoire cloning to recover Fab fragments from bone marrow mRNA of chimpanzees infected ... More
    Molecular genetics of tetrahydrobiopterin (BH4) deficiency in the Maltese population.
    Authors:Farrugia R; Scerri CA; Montalto SA; Parascandolo R; Neville BG; Felice AE
    Journal:Molecular Genetics and Metabolism
    PubMed ID:
    Deficient activity of the Dihydropteridine Reductase enzyme (DHPR; EC 1.5.1.34; OMIM 261630) is due to mutations in the Quinoid Dihydropteridine Reductase gene on 4p15.3 (QDPR; RefSeq NM_000320). It results in defective recycling of tetrahydrobiopterin (BH(4)) and homozygotes have a rare form of atypical Hyperphenylalaninaemia and Phenylketonuria (aPKU). The heterozygote frequency ... More
    A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast.
    Authors:Eisenkolb M; Zenzmaier C; Leitner E; Schneiter R
    Journal:Molecular Biology of the Cell
    PubMed ID:
    Fungal sphingolipids contain ceramide with a very-long-chain fatty acid (C26). To investigate the physiological significance of the C26-substitution on this lipid, we performed a screen for mutants that are synthetically lethal with ELO3. Elo3p is a component of the ER-associated fatty acid elongase and is required for the final elongation ... More
    Procollagen with skipping of alpha 1(I) exon 41 has lower binding affinity for alpha 1(I) C-telopeptide, impaired in vitro fibrillogenesis, and altered fibril morphology.
    Authors:Cabral WA; Fertala A; Green LK; Korkko J; Forlino A; Marini JC
    Journal:The Journal of Biological Chemistry
    PubMed ID:
    Previous in vitro data on type I collagen self-assembly into fibrils suggested that the amino acid 776-796 region of the alpha1(I) chain is crucial for fibril formation because it serves as the recognition site for the telopeptide of a docking collagen monomer. We used a natural collagen mutation with a ... More
    Vibrio fischeri LuxS and AinS: comparative study of two signal synthases.
    Authors:Lupp C; Ruby EG
    Journal:Journal of Bacteriology
    PubMed ID:
    Vibrio fischeri possesses two acyl-homoserine lactone quorum-sensing systems, ain and lux, both of which are involved in the regulation of luminescence gene expression and are required for persistent colonization of the squid host, Euprymna scolopes. We have previously demonstrated that the ain system induces luminescence at cell densities that precede ... More