A prelysosomal compartment sequesters membrane-impermeant fluorescent dyes from the cytoplasmic matrix of J774 macrophages.
AuthorsSteinberg TH, Swanson JA, Silverstein SC
JournalJ Cell Biol
PubMed ID3417786
'After the membrane impermeant dye Lucifer Yellow is introduced into the cytoplasmic matrix of J774 cells, the dye is sequestered within cytoplasmic vacuoles and secreted into the extracellular medium. In the present work we studied the intracellular transport of Lucifer Yellow in J774 macrophages and the nature of the cytoplasmic ... More
Identification of Legionella pneumophila mutants that have aberrant intracellular fates.
AuthorsSwanson MS, Isberg RR
JournalInfect Immun
PubMed ID8698483
'After uptake by macrophages, Legionella pneumiophila evades phagosome-lysosome fusion and replicates in a compartment associated with the endoplasmic reticulum. A collection of bacterial mutants defective for growth in macrophages were isolated, and the intracellular fate of each mutant strain was analyzed by fluorescence microscopy. To measure intracellular replication, bacteria inside ... More
Involvement of early and late lysosomes in the degradation of mannosylated ligands by rat liver endothelial cells.
AuthorsKjeken R, Brech A, Løvdal T, Roos N, Berg T
JournalExp Cell Res
PubMed ID7843273
'The intracellular transport and degradation of endocytosed mannosylated albumin (Man-BSA) was studied in cell cultures of rat liver endothelial cells by subcellular fractionation, fluorescence microscopy, and electron microscopy. The ligand used for subcellular fractionation experiments was labeled with 125I-labeled tyramine cellobiose or 131I-labeled tyramine cellobiose. The labeled degradation products are ... More
Analysis of the intracellular fate of Legionella pneumophila mutants.
AuthorsSwanson MS, Isberg RR
JournalAnn N Y Acad Sci
PubMed ID8993346
'L. pneumophila is a model organism for investigating the mechanisms by which intracellular pathogens acquire the metabolites needed for replication while evading the microbicidal mechanisms of the macrophage. We determined that intracellular L. pneumophila replicate in close association with the endoplasmic reticulum and suggest that L. pneumophila exploits the macrophage ... More
Fluorescent labeling of endocytic compartments.
AuthorsSwanson J
JournalMethods Cell Biol
PubMed ID2464119
Cycling of the integral membrane glycoprotein, LEP100, between plasma membrane and lysosomes: kinetic and morphological analysis.
AuthorsLippincott-Schwartz J, Fambrough DM
JournalCell
PubMed ID3107839
'LEP100 (an integral membrane glycoprotein, Mr = 100,000) occurs in three subcellular compartments: lysosome (approximately 90% of the molecules), endosome (5%-8%), and plasma membrane (2%-3%). Rate constants for movement to and from each compartment have been estimated. The movement of LEP100 from endosomes to lysosomes was blocked by chloroquine, causing ... More
Toxoplasma gondii: fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts.
AuthorsJoiner KA, Fuhrman SA, Miettinen HM, Kasper LH, Mellman I
JournalScience
PubMed ID2200126
After actively entering its host cells, the protozoan parasite Toxoplasma gondii resides in an intracellular vacuole that is completely unable to fuse with other endocytic or biosynthetic organelles. The fusion blocking requires entry of viable organisms but is irreversible: fusion competence of the vacuole is not restored if the parasite ... More
The parasitophorous vacuole membrane surrounding intracellular Toxoplasma gondii functions as a molecular sieve.
AuthorsSchwab JC, Beckers CJ, Joiner KA
JournalProc Natl Acad Sci U S A
PubMed ID8290555
The obligate intracellular protozoan parasite Toxoplasma gondii creates and enters into a unique membrane-bounded cytoplasmic compartment, the parasitophorous vacuole, when invading mammalian host cells. By microinjecting polar fluorescent molecules into individual T. gondii-infected fibroblasts, we show here that the parasitophorous vacuole membrane (PVM) surrounding the parasite functions as a molecular ... More
Visualization of peptides derived from liposome-encapsulated proteins in the trans-Golgi area of macrophages.
AuthorsRao M, Rothwell SW, Wassef NM, Pagano RE, Alving CR
JournalImmunol Lett
PubMed ID9373218
Exogenous proteins are generally not presented through the major histocompatibility complex (MHC) class I pathway, yet several recent studies show that particle-associated antigens induce a CD8+ T-cell response. Therefore, a pathway must exist in vivo for the presentation of exogenous antigens on class I molecules. In the present study, we ... More
Characterization of endoplasmic reticulum by co-localization of BiP and dicarbocyanine dyes.
AuthorsTerasaki M, Reese TS
JournalJ Cell Sci
PubMed ID1378452
The original concept of endoplasmic reticulum derived from the observation of a reticular network in cultured fibroblasts by electron microscopy of whole cells. It was previously reported that the fluorescent dye, DiOC6(3), stains a similar network as well as mitochondria and other organelles in living cells. Here, we investigate the ... More
EEA1, a tethering protein of the early sorting endosome, shows a polarized distribution in hippocampal neurons, epithelial cells, and fibroblasts.
AuthorsWilson JM, de Hoop M, Zorzi N, Toh BH, Dotti CG, Parton RG
JournalMol Biol Cell
PubMed ID10930461
EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes. Because of the presence of complex endosomal pathways in polarized and nonpolarized cells, we have examined the distribution of EEA1 in diverse cell types. Ultrastructural analysis ... More
Accumulation of membrane glycoproteins in lysosomes requires a tyrosine residue at a particular position in the cytoplasmic tail.
AuthorsWilliams MA, Fukuda M
JournalJ Cell Biol
PubMed ID2391371
Human lysosome membrane glycoprotein h-lamp-1 is a highly N-glycosylated protein found predominantly in lysosomes, with low levels present at the cell surface. The signal required for delivery of h-lamp-1 to lysosomes was investigated by analyzing the intracellular distribution of h-lamp-1 with altered amino acid sequences expressed from mutated cDNA clones. ... More
Legionella pneumophila replication vacuoles mature into acidic, endocytic organelles.
AuthorsSturgill-Koszycki S, Swanson MS
JournalJ Exp Med
PubMed ID11067875
After ingestion by macrophages, Legionella pneumophila inhibits acidification and maturation of its phagosome. After a 6-10-h lag period, the bacteria replicate for 10-14 h until macrophage lysis releases dozens of progeny. To examine whether the growth phase of intracellular L. pneumophila determines the fate of its phagosome, interactions between the ... More
Association of the invariant chain with major histocompatibility complex class I molecules directs trafficking to endocytic compartments.
AuthorsSugita M, Brenner MB
JournalJ Biol Chem
PubMed ID7836413
Major histocompatibility complex (MHC) class I and class II molecules have been shown to present peptides of different origin to alpha beta T cells. Most peptides presented by class I molecules are derived from endogenously synthesized proteins, whereas most peptides presented by class II molecules are from exogenous sources. This ... More
Mycobacterial infection of macrophages results in membrane-permeable phagosomes.
Cell-mediated immunity is critical for host resistance to tuberculosis. T lymphocytes recognizing antigens presented by the major histocompatibility complex (MHC) class I and class II molecules have been found to be necessary for control of mycobacterial infection. Mice genetically deficient in the generation of MHC class I and class Ia ... More
Evidence that secretase cleavage of cell surface Alzheimer amyloid precursor occurs after normal endocytic internalization.
AuthorsRefolo LM, Sambamurti K, Efthimiopoulos S, Pappolla MA, Robakis NK
JournalJ Neurosci Res
PubMed ID7602619
Three different treatments (methylamine, colchicine, and 18 degrees C temperature block), known to disrupt normal endocytic internalization, significantly reduced the secretory cleavage of cell surface-derived Alzheimer amyloid precursor (APP) in non-transfected C6 cell cultures. Conversely, treatments with methylamine or colchicine had no significant effect on the secretion of total APP. ... More
Targeting of an intestinal apical endosomal protein to endosomes in nonpolarized cells.
AuthorsWilson JM, Colton TL
JournalJ Cell Biol
PubMed ID9015303
Polarized cells such as epithelial cells and neurons have distinct endosomal compartments associated with different plasma membrane domains. The endosomes of the neuronal cell body and the basolateral cytoplasm of epithelial cells are thought to perform cellular "housekeeping" functions such as the uptake of nutrients and metabolites, while the endosomes ... More
Products of endocytosis and autophagy are retrieved from axons by regulated retrograde organelle transport.
AuthorsHollenbeck PJ
JournalJ Cell Biol
PubMed ID7682217
Cellular homeostasis in neurons requires that the synthesis and anterograde axonal transport of protein and membrane be balanced by their degradation and retrograde transport. To address the nature and regulation of retrograde transport in cultured sympathetic neurons, I analyzed the behavior, composition, and ultrastructure of a class of large, phase-dense ... More
Intracellular maturation and transport of the SV5 type II glycoprotein hemagglutinin-neuraminidase: specific and transient association with GRP78-BiP in the endoplasmic reticulum and extensive internalization from the cell surface.
AuthorsNg DT, Randall RE, Lamb RA
JournalJ Cell Biol
PubMed ID2557352
The hemagglutinin-neuraminidase (HN) glycoprotein of the paramyxovirus SV5 is a type II integral membrane protein that is expressed at the infected cell surface. The intracellular assembly and transport of HN in CV1 cells was examined using conformation-specific HN mAbs and sucrose density sedimentation analysis. HN was found to oligomerize with ... More
Trafficking of cell surface beta-amyloid precursor protein: retrograde and transcytotic transport in cultured neurons.
AuthorsYamazaki T, Selkoe DJ, Koo EH
JournalJ Cell Biol
PubMed ID7721945
Amyloid beta-protein (A beta), the principal constituent of senile plaques seen in Alzheimer's disease (AD), is derived by proteolysis from the beta-amyloid precursor protein (beta PP). The mechanism of A beta production in neurons, which are hypothesized to be a rich source of A beta in brain, remains to be ... More
Prediction of permeability-surface area product data by continuous-distribution pore models.
AuthorsMcNamee JE, Wolf MB
JournalMicrocirculation
PubMed ID9866118
OBJECTIVE: To investigate the ability of continuous-distribution pore models to accurately predict permeability-surface area product (PS) experimental data in skeletal muscle. METHODS: Models having a water-only (WO) pathway and continuous distributions of microvascular transport-pathway sizes were fit to solute reflection-coefficient (sigma) experimental data (approximately 0.5-16 nm Stokes radii) obtained from ... More
Diffusion of albumins in rat cortical slices and relevance to volume transmission.
AuthorsTao L, Nicholson C
JournalNeuroscience
PubMed ID8951877
The apparent diffusion coefficient, D*, was measured in rat cortical slices and compared to the free diffusion coefficient, D, for three negatively charged proteins, lactalbumin (mol. wt = 14,500), ovalbumin (45,000) and bovine serum albumin (66,000). The temporal evolution of the spatial distribution of albumin molecules labeled with the Texas ... More
The paramyxovirus simian virus 5 hemagglutinin-neuraminidase glycoprotein, but not the fusion glycoprotein, is internalized via coated pits and enters the endocytic pathway.
AuthorsLeser GP, Ector KJ, Lamb RA
JournalMol Biol Cell
PubMed ID8741847
The hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of the paramyxovirus simian virus 5 (SV5) are expressed on the surface of virus-infected cells. Although the F protein was found to be expressed stably, the HN protein was internalized from the plasma membrane. HN protein lacks known internalization signals in its cytoplasmic ... More
Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis.
Authors
JournalElife
PubMed ID34677124
Facile assembly and loading of theranostic polymersomes via multi-impingement flash nanoprecipitation.