Oregon Green™ 488-X, Succinimidyl Ester, 6-isomer - Citations

Oregon Green™ 488-X, Succinimidyl Ester, 6-isomer - Citations

View additional product information for Oregon Green™ 488-X, Succinimidyl Ester, 6-isomer - Citations (O6185)

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Abstract
Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores.
AuthorsRozinov MN, Nolan GP
JournalChem Biol
PubMed ID9862799
'BACKGROUND: Fluorophore dyes are used extensively in biomedical research to sensitively assay cellular constituents and physiology. We have created, as proof of principle, fluorophore dye binding peptides that could have applications in fluorescent dye-based approaches in vitro and in vivo. RESULTS: A panel of Texas red, Rhodamine red, Oregon green ... More
Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. I. Chemical synthesis of various reporter group-labeled 2'-deoxyribonucleoside-5'-triphosphates.
AuthorsGiller G, Tasara T, Angerer B, Mühlegger K, Amacker M, Winter H
JournalNucleic Acids Res
PubMed ID12736313
Fluorescent-labeled DNA is generated through enzymatic incorporation of fluorophore-linked 2'-deoxyribonucleoside-5'-triphosphates (dNTPs) by DNA polymerases. We describe the synthesis of a variety of dye-labeled dNTPs. Amino-linker-modified 5'-triphosphates of all four naturally occurring nucleobases were used as precursors. Commercially available dyes were coupled to the amino function of the side chain. In ... More
Cystic fibrosis transmembrane conductance regulator-independent phagosomal acidification in macrophages.
AuthorsHaggie PM, Verkman AS,
JournalJ Biol Chem
PubMed ID17724021
It was reported recently that the cystic fibrosis transmembrane conductance regulator (CFTR) is required for acidification of phagosomes in alveolar macrophages (Di, A., Brown, M. E., Deriy, L. V., Li, C., Szeto, F. L., Chen, Y., Huang, P., Tong, J., Naren, A. P., Bindokas, V., Palfrey, H. C., and Nelson, ... More
Noninvasive imaging of cell death using an Hsp90 ligand.
AuthorsPark D, Don AS, Massamiri T, Karwa A, Warner B, MacDonald J, Hemenway C, Naik A, Kuan KT, Dilda PJ, Wong JW, Camphausen K, Chinen L, Dyszlewski M, Hogg PJ,
JournalJ Am Chem Soc
PubMed ID21322555
Cell death plays a central role in normal physiology and in disease. Common to apoptotic and necrotic cell death is the eventual loss of plasma membrane integrity. We have produced a small organoarsenical compound, 4-(N-(S-glutathionylacetyl)amino)phenylarsonous acid, that rapidly accumulates in the cytosol of dying cells coincident with loss of plasma ... More
Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase.
AuthorsGoodall JJ, Chen GJ, Page MG
JournalBiochemistry
PubMed ID15078105
The peptidyl-tRNA hydrolase (Pth) enzyme plays an essential role in recycling tRNA from peptidyl-tRNA that has prematurely dissociated from the ribosome. In this study of Escherichia coli Pth, the critical role of histidine 20 was investigated by site-directed mutagenesis, stopped-flow kinetic measurements, and chemical modification. The histidine residue at position ... More
Real-time assessment of spatial and temporal coupled catalysis within polyelectrolyte microcapsules containing coimmobilized glucose oxidase and peroxidase.
AuthorsStein EW, Volodkin DV, McShane MJ, Sukhorukov GB
JournalBiomacromolecules
PubMed ID16529405
The encapsulation of biological enzymes within polyelectrolyte microcapsules is an important step toward microscale devices for processing and analytical applications, one which could be applied to the realization of minimally invasive sensing technology. In this work, the encapsulation and functional characterization of a bienzymatic coupled catalytic system within polyelectrolyte microcapsules ... More