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Oregon Green® 488 BAPTA-1 dextran, Potassium Salt, 10,000 MW, Anionic

Catalog number: O6798

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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Description

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissues. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated indicators using patch pipette, microinjection, or our Influx pinocyte-loading reagent. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms.

Calcium Indicator (Cell-Impermeant Salts, Dextran Conjugates) Specifications:
• Label (Ex/Em): Oregon Green® 488 BAPTA-1 (494/523 nm)
• Fluorescence intensity increase upon binding Ca2+: ~14 fold
• Dextran size: 10,000 MW
• Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength


Spectral Characteristics of Molecular Probes® Calcium Indicators
These probes are excited by visible light, and because the energy required for excitation is low, the potential for cellular photodamage is reduced. Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of the spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem.

More Choices for Fluorescent Calcium Indicators
We offer a large selection of Molecular Probes® calcium indicators for use in various experimental scenarios, for example dextran versions for reduced leakage and compartmentalization and BAPTA conjugates for detecting high-amplitude calcium transients. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes® Handbook.

For UV-excitable Ca2+ indicators, protein-based Ca2+ indicators, conjugates of Ca2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg2+, Zn2+) review Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: Calcium Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Microplate Reader, Fluorescence Microscope, Flow Cytometer
Product Size: 5 mg
Detection Method: Fluorescent
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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Manuals & protocols

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