Oregon Green™ 488 BAPTA-5N, Hexapotassium Salt, cell impermeant
Oregon Green™ 488 BAPTA-5N, Hexapotassium Salt, cell impermeant
Citas y referencias (46)
Invitrogen™

Oregon Green™ 488 BAPTA-5N, Hexapotassium Salt, cell impermeant

El indicador de Ca2+ de baja afinidad, excitable con luz visible e impenetrable en las células, Oregon Green™ 488 BAPTA-5N,Más información
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Número de catálogoCantidad
O6812500 μg
Número de catálogo O6812
Precio (MXN)
-
Cantidad:
500 μg
El indicador de Ca2+ de baja afinidad, excitable con luz visible e impenetrable en las células,

Oregon Green™ 488 BAPTA-5N, sal de hexapotásico
tiene una Kd para Ca2+ de ∼20 µM y una absorción/emisión máxima de ∼492/517 nm.

Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de la membrana ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteA base de colorantes fluorescentes
Cantidad500 μg
Condiciones de envíoTemperatura ambiente
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo, Lector de microplacas
Línea de productosOregon Green
Tipo de productoIndicador de calcio
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Citations & References (46)

Citations & References
Abstract
Presynaptic Ca(2+) influx at the inhibitor of the crayfish neuromuscular junction: a photometric study at a high time resolution.
Authors:Vyshedskiy A, Lin JW
Journal:J Neurophysiol
PubMed ID:10634895
'Presynaptic calcium influx at the inhibitor of the crayfish neuromuscular junction was investigated by measuring fluorescence transients generated by calcium-sensitive dyes. This approach allowed us to correlate presynaptic calcium influx with transmitter release at a high time resolution. Systematic testing of the calcium indicators showed that only low-affinity dyes, with ... More
Light dependence of calcium and membrane potential measured in blowfly photoreceptors in vivo.
Authors:Oberwinkler J, Stavenga DG
Journal:J Gen Physiol
PubMed ID:9689022
'Light adaptation in insect photoreceptors is caused by an increase in the cytosolic Ca2+ concentration. To better understand this process, we measured the cytosolic Ca2+ concentration in vivo as a function of adapting light intensity in the white-eyed blowfly mutant chalky. We developed a technique to measure the cytosolic Ca2+ ... More
Stimulation-evoked increases in cytosolic [Ca(2+)] in mouse motor nerve terminals are limited by mitochondrial uptake and are temperature-dependent.
Authors:David G, Barrett EF
Journal:J Neurosci
PubMed ID:11007886
'Increases in cytosolic [Ca(2+)] evoked by trains of action potentials (20-100 Hz) were recorded from mouse and lizard motor nerve terminals filled with a low-affinity fluorescent indicator, Oregon Green BAPTA 5N. In mouse terminals at near-physiological temperatures (30-38 degrees C), trains of action potentials at 25-100 Hz elicited increases in ... More
Direct measurement of SR release flux by tracking 'Ca2+ spikes' in rat cardiac myocytes.
Authors:Song LS, Sham JS, Stern MD, Lakatta EG, Cheng H
Journal:J Physiol
PubMed ID:9769413
'1. Ca2+ release flux across the sarcoplasmic reticulum (SR) during cardiac excitation-contraction coupling was investigated using a novel fluorescence method. Under whole-cell voltage-clamp conditions, rat ventricular myocytes were dialysed with a high concentration of EGTA (4.0 mM, 150 nM free Ca2+), to minimize the residence time of released Ca2+ in ... More
Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells.
Authors:Sherwood MW, Prior IA, Voronina SG, Barrow SL, Woodsmith JD, Gerasimenko OV, Petersen OH, Tepikin AV
Journal:Proc Natl Acad Sci U S A
PubMed ID:17363470
'The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, is an important early step in the development of acute pancreatitis. The cellular compartment in which trypsinogen activation occurs currently is unknown. We therefore investigated the site of intracellular trypsinogen activation by using an established cellular model of acute ... More
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