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Citations & References (28)
Invitrogen™
Phen Green™ SK, Diacetate
Green-fluorescent heavy metal indicator can be used to detect a broad range of ions
| Catalog Number | Quantity |
|---|---|
| P14313 | 1 mg |
Catalog number P14313
Price (USD)
415.00
Each
Quantity:
1 mg
Price (USD)
415.00
Each
The green-fluorescent heavy metal indicator Phen Green™ SK, diacetate can be used to detect a broad range of ions, including Cu2+, Cu+, Fe2+, Hg2+, Pb2+, Cd2+, Zn2+ and Ni2+.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypePhen Green
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Application)Heavy Metal Indicator
For Use With (Equipment)Fluorescence Microscope, Microplate Reader
Product LinePhen Green
Product TypeDiacetate
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C.
Citations & References (28)
Citations & References
Abstract
Determination of the chelatable iron pool of single intact cells by laser scanning microscopy.
Journal:Arch Biochem Biophys
PubMed ID:10729192
'We have previously established a method of detecting intracellular chelatable iron in viable cells based on digital fluorescence microscopy. To quantify cellular chelatable iron, it was crucial to determine the intracellular indicator concentration. In the present study, we therefore adapted the method to confocal laser scanning microscopy, which should allow
Cold-induced apoptosis of hepatocytes: mitochondrial permeability transition triggered by nonmitochondrial chelatable iron.
Journal:Free Radic Biol Med
PubMed ID:14680689
'We previously described that the cold-induced apoptosis of cultured hepatocytes is mediated by an increase in the cellular chelatable iron pool. We here set out to assess whether a mitochondrial permeability transition (MPT) is involved in cold-induced apoptosis. When cultured hepatocytes were rewarmed after 18 h of cold (4 degrees
Copper transport across pea thylakoid membranes.
Journal:Plant Physiol
PubMed ID:15122011
'The initial rate of Cu2+ movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu(2+)-sensitive fluorophore Phen Green SK. Cu2+ transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu2+ concentration
Determination of the chelatable iron pool of isolated rat hepatocytes by digital fluorescence microscopy using the fluorescent probe, phen green SK.
Journal:Hepatology
PubMed ID:10094962
'The intracellular pool of chelatable iron is considered to be a decisive pathogenetic factor for various kinds of cell injury. We therefore set about establishing a method of detecting chelatable iron in isolated hepatocytes based on digital fluorescence microscopy. The fluorescence of hepatocytes loaded with the fluorescent metal indicators, phen
The relationship between intracellular free iron and cell injury in cultured neurons, astrocytes, and oligodendrocytes.
Journal:J Neurosci
PubMed ID:12122047
'Iron is an essential element for cells but may also be an important cytotoxin. However, very little is known about iron transport, redox status, or toxicity specifically inside cells. In this study, we exploited the sensitivity of fura-2 to quenching by ferrous iron (Fe(2+)) to detect intracellular free iron ([Fe(2+)](i))