Determination of the chelatable iron pool of single intact cells by laser scanning microscopy.
AuthorsPetrat F, de Groot H, Rauen U
JournalArch Biochem Biophys
PubMed ID10729192
'We have previously established a method of detecting intracellular chelatable iron in viable cells based on digital fluorescence microscopy. To quantify cellular chelatable iron, it was crucial to determine the intracellular indicator concentration. In the present study, we therefore adapted the method to confocal laser scanning microscopy, which should allow ... More
Cold-induced apoptosis of hepatocytes: mitochondrial permeability transition triggered by nonmitochondrial chelatable iron.
AuthorsRauen U, Kerkweg U, Weisheit D, Petrat F, Sustmann R, de Groot H
JournalFree Radic Biol Med
PubMed ID14680689
'We previously described that the cold-induced apoptosis of cultured hepatocytes is mediated by an increase in the cellular chelatable iron pool. We here set out to assess whether a mitochondrial permeability transition (MPT) is involved in cold-induced apoptosis. When cultured hepatocytes were rewarmed after 18 h of cold (4 degrees ... More
Copper transport across pea thylakoid membranes.
AuthorsShingles R, Wimmers LE, McCarty RE
JournalPlant Physiol
PubMed ID15122011
'The initial rate of Cu2+ movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu(2+)-sensitive fluorophore Phen Green SK. Cu2+ transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu2+ concentration ... More
Determination of the chelatable iron pool of isolated rat hepatocytes by digital fluorescence microscopy using the fluorescent probe, phen green SK.
AuthorsPetrat F, Rauen U, de Groot H
JournalHepatology
PubMed ID10094962
'The intracellular pool of chelatable iron is considered to be a decisive pathogenetic factor for various kinds of cell injury. We therefore set about establishing a method of detecting chelatable iron in isolated hepatocytes based on digital fluorescence microscopy. The fluorescence of hepatocytes loaded with the fluorescent metal indicators, phen ... More
The relationship between intracellular free iron and cell injury in cultured neurons, astrocytes, and oligodendrocytes.
AuthorsKress GJ, Dineley KE, Reynolds IJ
JournalJ Neurosci
PubMed ID12122047
'Iron is an essential element for cells but may also be an important cytotoxin. However, very little is known about iron transport, redox status, or toxicity specifically inside cells. In this study, we exploited the sensitivity of fura-2 to quenching by ferrous iron (Fe(2+)) to detect intracellular free iron ([Fe(2+)](i)) ... More
Time to fatigue is increased in mouse muscle at 37 degrees C; the role of iron and reactive oxygen species.
AuthorsReardon TF, Allen DG,
JournalJ Physiol
PubMed ID19470779
'Studies exploring the rate of fatigue in isolated muscle at 37 degrees C have produced mixed results. In the present study, muscle fibre bundles from the mouse foot were used to study the effect of temperature on the rate of muscle fatigue. Provided iron was excluded from the solutions, time ... More
Chronic exposure to nitric oxide alters the free iron pool in endothelial cells: role of mitochondrial respiratory complexes and heat shock proteins.
AuthorsRamachandran A, Ceaser E, Darley-Usmar VM
JournalProc Natl Acad Sci U S A
PubMed ID14691259
'The mechanisms of nitric oxide (NO) signaling include binding to the iron centers in soluble guanylate cyclase and cytochrome c oxidase and posttranslational modification of proteins by S-nitrosation. Low levels of NO control mitochondrial number in cells, but little is known of the impact of chronic exposure to high levels ... More
Metals in neurobiology: probing their chemistry and biology with molecular imaging.
AuthorsQue EL, Domaille DW, Chang CJ,
JournalChem Rev
PubMed ID18426241
'In this review, we will provide a brief overview of the field of transition metals in neurobiology, focusing on the contributions of d-block metals zinc, iron, and copper to neurophysiology, aging, and neuropathology, as well as progress in the development of molecular probes for visualizing zinc, iron, and copper ion ... More
Design and applications of methods for fluorescence detection of iron in biological systems.
AuthorsEspósito BP, Breuer W, Cabantchik ZI
JournalBiochem Soc Trans
PubMed ID12196179
'Fluorescence metalosensors provide a means to detect iron in biological systems that is versatile, economical, sensitive and of a high-throughput nature. They rely on relatively high-affinity iron-binding carriers conjugated to highly fluorescent probes that undergo quenching after metal complexation. Metal specificity is determined by probes containing either an iron-binding moiety ... More
Copper transport by lobster (Homarus americanus) hepatopancreatic lysosomes.
AuthorsChavez-Crooker P, Garrido N, Pozo P, Ahearn GA
JournalComp Biochem Physiol C Toxicol Pharmacol
PubMed ID12860049
'Lysosomes are known centers for sequestration of calcium and a variety of heavy metals in many invertebrate tissues, and as a result of this compartmentalization these organelles perform important detoxification roles in the animals involved. The present investigation uses a centrifugation method to isolate and purify hepatopancreatic lysosomes from the ... More
A review of fluorescence methods for assessing labile iron in cells and biological fluids.
AuthorsEspósito BP, Epsztejn S, Breuer W, Cabantchik ZI
JournalAnal Biochem
PubMed ID11969183
A variety of biochemical, pharmacological, and toxicological properties have been attributed to labile forms of iron that are associated with cells or with biological fluids. Unlike the major fraction of bioiron which is protein bound, the labile bioiron is chelatable and therefore amenable for detection by metal-sensing devices that are ... More
Ferrous ion transport across chloroplast inner envelope membranes.
AuthorsShingles R, North M, McCarty RE
JournalPlant Physiol
PubMed ID11891257
The initial rate of Fe(2+) movement across the inner envelope membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membrane vesicles loaded with the Fe(2+)-sensitive fluorophore, Phen Green SK. The rate of Fe(2+) transport was rapid, coming to equilibrium within 3s. The maximal rate and concentration ... More
Fluorescence detection of redox-sensitive metals in neuronal culture: focus on iron and zinc.
AuthorsReynolds IJ
JournalAnn N Y Acad Sci
PubMed ID15105253
Detection of neurotoxic metals in the intracellular milieu has made an important contribution to the understanding of the mechanism of metal-induced neuronal injury. Fluorescent, metal-sensitive dyes have proven to be valuable in the measurement of a variety of neurotoxic cations in neurons, and these dyes have provided a number of ... More
Subcellular distribution of chelatable iron: a laser scanning microscopic study in isolated hepatocytes and liver endothelial cells.
AuthorsPetrat F, de Groot H, Rauen U
JournalBiochem J
PubMed ID11336636
The pool of cellular chelatable iron ('free iron', 'low-molecular-weight iron', the 'labile iron pool') is usually considered to reside mainly within the cytosol. For the present study we adapted our previously established Phen Green method, based on quantitative laser scanning microscopy, to examine the subcellular distribution of chelatable iron in ... More
Copper transport by lobster (Homarus americanus) hepatopancreatic mitochondria.
AuthorsChavez-Crooker P, Garrido N, Ahearn GA
JournalJ Exp Biol
PubMed ID11854377
Mechanisms of copper transport into purified mitochondrial suspensions prepared from the hepatopancreas of the Atlantic lobster Homarus americanus were investigated. Mitochondria were purified by combining methods of differential and Percoll-gradient centrifugation, and copper transport was studied using the copper-sensitive fluorescent dye Phen Green. Copper transport by this mitochondrial preparation was ... More
Enhancement of iron toxicity in L929 cells by D-glucose: accelerated(re-)reduction.
AuthorsLehnen-Beyel I, Groot HD, Rauen U
JournalBiochem J
PubMed ID12193041
It has recently been shown that an increase in the cellular chelatable iron pool is sufficient to cause cell damage. To further characterize this kind of injury, we artificially enhanced the chelatable iron pool in L929 mouse fibroblasts using the highly membrane-permeable complex Fe(III)/8-hydroxyquinoline. This iron complex induced a significant ... More
Hypothermia injury/cold-induced apoptosis--evidence of an increase in chelatable iron causing oxidative injury in spite of low O2-/H2O2 formation.
AuthorsRauen U, Petrat F, Li T, De Groot H
JournalFASEB J
PubMed ID11023979
When incubated at 4 degrees C, cultured rat hepatocytes or liver endothelial cells exhibit pronounced injury and, during earlier rewarming, marked apoptosis. Both processes are mediated by reactive oxygen species, and marked protective effects of iron chelators as well as the protection provided by various other antioxidants suggest that hydroxyl ... More
Serofendic acid, a sulfur-containing diterpenoid derived from fetal calf serum, attenuates reactive oxygen species-induced oxidative stress in cultured striatal neurons.
AuthorsOsakada F, Kawato Y, Kume T, Katsuki H, Sugimoto H, Akaike A
JournalJ Pharmacol Exp Ther
PubMed ID15159446
We previously identified a novel endogenous substance, serofendic acid, from a lipophilic extract of fetal calf serum. Serofendic acid protects cultured cortical neurons against the cytotoxicity of glutamate and nitric oxide. Here, we reported the protective effect of serofendic acid on reactive oxygen species-induced oxidative stress using primary rat striatal ... More
Ferroptosis inhibitor ferrostatin‑1 alleviates homocysteine‑induced ovarian granulosa cell injury by regulating TET activity and DNA methylation.
Authors
JournalMol Med Rep
PubMed ID35169856
Steap4 plays a critical role in osteoclastogenesis in vitro by regulating cellular iron/reactive oxygen species (ROS) levels and cAMP response element-binding protein (CREB) activation.
Authors
JournalJ Biol Chem
PubMed ID23990467
Interactions of Potential Anti-COVID-19 Compounds with Multispecific ABC and OATP Drug Transporters.
Authors
JournalPharmaceutics
PubMed ID33435273
Natural Product Erianin Inhibits Bladder Cancer Cell Growth by Inducing Ferroptosis via NRF2 Inactivation.
Authors
JournalFront Pharmacol
PubMed ID34776986
The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice.
Authors
JournalElife
PubMed ID34762046
Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells.
Authors
JournalNutrients
PubMed ID30813537
Ferritinophagy is involved in the zinc oxide nanoparticles-induced ferroptosis of vascular endothelial cells.
Authors
JournalAutophagy
PubMed ID33843441
Epigenetic regulation of ferroptosis via ETS1/miR-23a-3p/ACSL4 axis mediates sorafenib resistance in human hepatocellular carcinoma.
Authors
JournalJ Exp Clin Cancer Res
PubMed ID34980204
Modulation of the Neuroprotective and Anti-inflammatory Activities of the Flavonol Fisetin by the Transition Metals Iron and Copper.
Authors
JournalAntioxidants (Basel)
PubMed ID33187316
CD36-mediated ferroptosis dampens intratumoral CD8+ T cell effector function and impairs their antitumor ability.