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Thermo Scientific™

PDK1

Catalog number:  P3001

Related applications:

Protein Assays and Analysis

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Description

PDK1 (3-phosphoinositide dependent kinase-1) is a serine⁄threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NP_002604
Assay Entry: Biochemical binding/kinetic, Biochemical phospho-specific activity (no antibody)
Kinase Group: Ser⁄Thr Kinases: Atypical
Shipping Condition: Dry Ice
System Compatibility: Zin-LYTE™, Omnia® Bulk Substrate
Target Entry: PDK1
Target Gene: 3-phosphoinositide dependent kinase-1
Expression System: Baculovirus
Protein Family: Kinases & Inhibitors
Protein Form: Full Length, Recombinant
Protein Subtype: Serine⁄Threonine Kinases
Research Category: Signal Transduction
Species: Human
Validated Application: Kinase Assay
Gene ID (Entrez): 5163, 5170
Molecular Weight: 66.9 kDa
Gene Alias: PDK-1
KinaseFamily: PDHK Kinase Family
Protein Tag: His Tag

Contents & storage

Storage Buffer: 50mM Tris, pH 7.5, 100mM NaCl, 0.5mM EGTA, 0.5mM EDTA, 0.05% Triton X-100, 5mM DTT, 20% glycerol
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.