A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures.
AuthorsDolman NJ, Kilgore JA, Davidson MW,
Journal
PubMed ID23835803
'Fluorescent labeling of vesicular structures in cultured cells, particularly for live cells, can be challenging for a number of reasons. The first challenge is to identify a reagent that will be specific enough where some structures have a number of potential reagents and others very few options. The emergence of ... More
TREM2 mutations implicated in neurodegeneration impair cell surface transport and phagocytosis.
AuthorsKleinberger G, Yamanishi Y, Suárez-Calvet M, Czirr E, Lohmann E, Cuyvers E, Struyfs H, Pettkus N, Wenninger-Weinzierl A, Mazaheri F, Tahirovic S, Lleó A, Alcolea D, Fortea J, Willem M, Lammich S, Molinuevo JL, Sánchez-Valle R, Antonell A, Ramirez A, Heneka MT, Sleegers K, van der Zee J, Martin JJ, Engelborghs S, Demirtas-Tatlidede A, Zetterberg H, Van Broeckhoven C, Gurvit H, Wyss-Coray T, Hardy J, Colonna M, Haass C,
Journal
PubMed ID24990881
'Genetic variants in the triggering receptor expressed on myeloid cells 2 (TREM2) have been linked to Nasu-Hakola disease, Alzheimer''s disease (AD), Parkinson''s disease, amyotrophic lateral sclerosis, frontotemporal dementia (FTD), and FTD-like syndrome without bone involvement. TREM2 is an innate immune receptor preferentially expressed by microglia and is involved in inflammation ... More
P2X4 receptor regulates alcohol-induced responses in microglia.
AuthorsGofman L, Cenna JM, Potula R
Journal
PubMed ID25135400
'Mounting evidence indicates that alcohol-induced neuropathology may result from multicellular responses in which microglia cells play a prominent role. Purinergic receptor signaling plays a key role in regulating microglial function and, more importantly, mediates alcohol-induced effects. Our findings demonstrate that alcohol increases expression of P2X4 receptor (P2X4R), which alters the ... More
Induction of trabecular meshwork cells from induced pluripotent stem cells.
Loss or dysfunction of trabecular meshwork (TM) cells has been associated with the development of pathologically elevated IOP, and it is conceivable that replacement of damaged TM cells could restore function to the TM. We propose that the use of TM-like cells derived from induced pluripotent stem cells (iPSCs) created ... More
Pathogenic bacterial species associated with endodontic infection evade innate immune control by disabling neutrophils.
AuthorsMatsui A, Jin JO, Johnston CD, Yamazaki H, Houri-Haddad Y, Rittling SR,
Journal
PubMed ID25024367
Endodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. To investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections ... More
Glutathione reductase is essential for host defense against bacterial infection.
AuthorsYan J, Ralston MM, Meng X, Bongiovanni KD, Jones AL, Benndorf R, Nelin LD, Joshua Frazier W, Rogers LK, Smith CV, Liu Y,
JournalFree Radic Biol Med
PubMed ID23623936
Glutathione reductase (Gsr) catalyzes the reduction of glutathione disulfide to glutathione, a major cellular antioxidant. We have recently shown that Gsr is essential for host defense against the gram-negative bacteria Escherichia coli in a mouse model of sepsis. Although we have demonstrated that Gsr is required for sustaining the oxidative ... More
Cyclo(Phe-Pro) Produced by the Human Pathogen Vibrio vulnificus Inhibits Host Innate Immune Responses through the NF-?B Pathway.
AuthorsKim K, Kim NJ, Kim SY, Kim IH, Kim KS, Lee GR
Journal
PubMed ID25561711
Cyclo(Phe-Pro) (cFP) is a secondary metabolite produced by certain bacteria and fungi. Although recent studies highlight the role of cFP in cell-to-cell communication by bacteria, its role in the context of the host immune response is poorly understood. In this study, we investigated the role of cFP produced by the ... More
Vibrio cholerae MARTX toxin heterologous translocation of beta-lactamase and roles of individual effector domains on cytoskeleton dynamics.
AuthorsDolores JS, Agarwal S, Egerer M, Satchell KJ
Journal
PubMed ID25427654
The Vibrio cholerae MARTXVc toxin delivers three effector domains to eukaryotic cells. To study toxin delivery and function of individual domains, the rtxA gene was modified to encode toxin with an in-frame beta-lactamase (Bla) fusion. The hybrid RtxA::Bla toxin was Type I secreted from bacteria; and then Bla was translocated ... More
N-truncation and pyroglutaminylation enhances the opsonizing capacity of Aß-peptides and facilitates phagocytosis by macrophages and microglia.
AuthorsCondic M, Oberstein TJ, Herrmann M, Reimann MC, Kornhuber J, Maler JM, Spitzer P
Journal
PubMed ID24876064
Abnormal accumulations of amyloid-ß (Aß)-peptides are one of the pathological hallmarks of Alzheimer's disease (AD). The precursor of the Aß-peptides, the amyloid precursor protein (APP), is also found in peripheral blood cells, but its function in these cells remains elusive. We previously observed that mononuclear phagocytes release Aß-peptides during activation ... More
Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung.
Authors
JournalNat Commun
PubMed ID31481690
The metalloproteinase ADAM10 requires its activity to sustain surface expression.
Authors
JournalCell Mol Life Sci
PubMed ID32372373
Properdin Deficiency Impairs Phagocytosis and Enhances Injury at Kidney Repair Phase Post Ischemia-Reperfusion.