Invitrogen™

pHrodo™ Green E. coli BioParticles™ Conjugate for Phagocytosis

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Description

New pH-sensitive pHrodo® Green dye conjugates, like our pHrodo® Red dye conjugates, give faster and more accurate results than any other phagocytosis assay. pHrodo® Green conjugates are non-fluorescent outside the cell at neutral pH, but fluoresce brightly green at acidic pH such as in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.

• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Enough to do 100 tests when using 100 µL in each well of 96-well plate
• Multiplex with other compatible dyes, such as red florescent protein, TMRM, NucBlue™ Hoechst, and CellROX® Deep Red

The fluorescence of the novel pHrodo® Green dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.

Use the ready-made pHrodo® Green E. coli BioParticles® Conjugate in imaging, high content screening, high throughput screening, and flow applications. pHrodo® Green dye is also available as a conjugate of zymosan BioParticles®, S. aureus BioParticles®, or dextran 10,000 MW. To create other conjugates, such as antibody conjugates, use pHrodo® Green STP ester or pHrodo® Green maleimide.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type: Bioparticle Conjugate
Color: Green
Detection Method: Fluorescent
Excitation/Emission: 509⁄533
For Use With (Equipment): Attune™ Acoustic Focusing Cytometer, Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, High Content Instrument, Flow Cytometer
Label Type: pH-Sensitive Dyes
Label or Dye: pHrodo™ Green
Shipping Condition: Room Temperature
Product Line: pHrodo™
Form: Solid
Cell Type: Mammalian
Species: E. coli

Contents & storage

Store at -20 °C, dessicate and protected from light.