Get up to 200X more sensitivity in ICC/IHC/FISH for more clarity and definition with new Tyramide SuperBoost™ kits

pHrodo® Green S. aureus Bioparticles® Conjugate for Phagocytosis

Catalog number: P35367

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

New pH-sensitive pHrodo® Green dye conjugates, like our pHrodo® Red dye conjugates, give faster and more accurate results than any other phagocytosis assay. pHrodo® Green conjugates are non-fluorescent outside the cell at neutral pH, but fluoresce brightly green at acidic pH such as in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.

• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Enough to do 100 tests when using 100 µL in each well of a 96-well plate
• Multiplex with other compatible dyes, such as red florescent protein, TMRM, NucBlue™ Hoechst, and CellROX® Deep Red

The fluorescence of the novel pHrodo® Green dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.

Use the ready-made pHrodo® Green S. aureus BioParticles® Conjugate in imaging, high content screening, high throughput screening, and flow applications. pHrodo® Green dye is also available as a conjugate of E. coli BioParticles®, zymosan BioParticles®, or dextran 10,000. To create other conjugates, such as antibody conjugates, use pHrodo® Green STP ester or pHrodo® Green maleimide.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Green
Species: S. aureus
Label or Dye: pHrodo™ Green
Product Size: 5 x 2 mg
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope, High Content Instrument, Confocal Microscope, Flow Cytometer, Floid® Cell Imaging System, Attune® Acoustic Focusing Cytometer
Excitation⁄Emission (nm): 509⁄533
Shipping Condition: Room Temperature

Contents & storage

Store at -20 °C, dessicate and protected from light.

Documents

Manuals & protocols

Recommended products