Invitrogen™

pHrodo™ BioParticles™ Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry

Catalog number: P35367
Invitrogen™

pHrodo™ BioParticles™ Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry

Catalog number: P35367
Achieve faster staining and more accurate results without the need for wash steps or quencher dye with pH-sensitive pHrodo BioParticles conjugates and phagocytosis kit for flow cytometry. pHrodo BioParticles conjugates for phagocytosis are non-fluorescent outside the cell at neutral pH but fluoresce brightly in acidic pH environments, such as those of endosomes and lysosomes. They can be used in imaging, HCA, and HTA applications. The pHrodo BioParticles phagocytosis kit offers rapid assessment of phagocytic activity in whole blood using flow cytometry.
 
Catalog Number
P35367
Unit Size
5 x 2 mg
Description
pHrodo™ Green S. aureus BioParticles™ Conjugate for Phagocytosis
Price (USD)
Price: 506.00
Online price: 479.65
Your price:
Availability
-
Full specifications
Quantity
ColorGreen
DescriptionpHrodo™ Green S. aureus BioParticles™ Conjugate for Phagocytosis
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Attune Acoustic Focusing Cytometer, Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, High Content Analysis Instrument, Flow Cytometer
Product TypeConjugate
Dye TypepH-Sensitive Dyes
Product LinepHrodo™
Quantity5 x 2 mg
Shipping ConditionRoom Temperature
SpeciesS. aureus
Excitation/Emission509/533
Unit Size5 x 2 mg
Catalog Number
Unit Size
5 x 2 mg
Description
pHrodo™ Deep Red E. coli BioParticles™ Conjugate for Phagocytosis
Price (USD)
Price: 562.00
Online price: 538.65
Your price:
Availability
-
Full specifications
Quantity
ColorDeep Red
DescriptionpHrodo™ Deep Red E. coli BioParticles™ Conjugate for Phagocytosis
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, High Content Analysis Instrument
Product TypeConjugate
Dye TypepHrodo™ Deep Red
FormLyophilized Powder
Quantity5 x 2 mg
Reagent TypeFluorogenic
SpeciesE. coli
Excitation/Emission640/655
Unit Size5 x 2 mg
Catalog Number
Unit Size
5 x 2 mg
Description
pHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis
Price (USD)
Price: 514.00
Online price: 472.65
Your price:
Availability
-
Full specifications
Quantity
ColorRed
DescriptionpHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope
Product TypeConjugate
Dye TypepHrodo™ Red
FormSolid
Product LineBioParticles™, pHrodo™
Quantity5 x 2 mg
Shipping ConditionRoom Temperature
SpeciesE. coli
Unit Size5 x 2 mg
Catalog Number
Unit Size
1 kit
Description
pHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry
Price (USD)
Price: 1,248.00
Online price: 1,144.65
Your price:
Availability
-
Full specifications
Quantity
ColorRed
DescriptionpHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Flow Cytometer
Product TypePhagocytosis Kit
Dye TypepHrodo™ Red
Emission488 nm
FormatBottle(s)
Product LineBioParticles™, pHrodo™
Quantity100 Assays
Shipping ConditionRoom Temperature
SpeciesE. coli
Unit Size1 kit
Catalog Number
Unit Size
5 x 2 mg
Description
pHrodo™ Red S. aureus BioParticles™ Conjugate for Phagocytosis
Price (USD)
Price: 508.00
Online price: 484.65
Your price:
Availability
-
Full specifications
Quantity
Cell TypeMammalian
ColorRed
DescriptionpHrodo™ Red S. aureus BioParticles™ Conjugate for Phagocytosis
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope
Product TypeConjugate
Dye TypepHrodo™ Red
FormSolid
Product LineBioParticles™, pHrodo™
Quantity5 x 2 mg
Shipping ConditionRoom Temperature
SpeciesS. aureus
Unit Size5 x 2 mg
Catalog Number
Unit Size
5 x 1 mg
Description
pHrodo™ Green Zymosan BioParticles™ Conjugate for Phagocytosis
Price (USD)
Price: 506.00
Online price: 466.65
Your price:
Availability
-
Full specifications
Quantity
ColorGreen
DescriptionpHrodo™ Green Zymosan BioParticles™ Conjugate for Phagocytosis
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Attune Acoustic Focusing Cytometer, Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, High Content Analysis Instrument, Flow Cytometer
Product TypeConjugate
Dye TypepH-Sensitive Dyes
Product LinepHrodo™
Quantity5 x 1 mg
Shipping ConditionRoom Temperature
SpeciesS. cerevisiae
Excitation/Emission509/533
Unit Size5 x 1 mg
Showing 6 of 6
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
P35367Full specifications
5 x 2 mgpHrodo™ Green S. aureus BioParticles™ Conjugate for Phagocytosis
Price: 506.00
Online price: 479.65
Your price:
-
ColorGreen
DescriptionpHrodo™ Green S. aureus BioParticles™ Conjugate for Phagocytosis
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Attune Acoustic Focusing Cytometer, Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, High Content Analysis Instrument, Flow Cytometer
Product TypeConjugate
Dye TypepH-Sensitive Dyes
Product LinepHrodo™
Quantity5 x 2 mg
Shipping ConditionRoom Temperature
SpeciesS. aureus
Excitation/Emission509/533
Unit Size5 x 2 mg
P35360Full specifications
5 x 2 mgpHrodo™ Deep Red E. coli BioParticles™ Conjugate for Phagocytosis
Price: 562.00
Online price: 538.65
Your price:
-
ColorDeep Red
DescriptionpHrodo™ Deep Red E. coli BioParticles™ Conjugate for Phagocytosis
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, High Content Analysis Instrument
Product TypeConjugate
Dye TypepHrodo™ Deep Red
FormLyophilized Powder
Quantity5 x 2 mg
Reagent TypeFluorogenic
SpeciesE. coli
Excitation/Emission640/655
Unit Size5 x 2 mg
P35361Full specifications
5 x 2 mgpHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis
Price: 514.00
Online price: 472.65
Your price:
-
ColorRed
DescriptionpHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope
Product TypeConjugate
Dye TypepHrodo™ Red
FormSolid
Product LineBioParticles™, pHrodo™
Quantity5 x 2 mg
Shipping ConditionRoom Temperature
SpeciesE. coli
Unit Size5 x 2 mg
A10025Full specifications
1 kitpHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry
Price: 1,248.00
Online price: 1,144.65
Your price:
-
ColorRed
DescriptionpHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Flow Cytometer
Product TypePhagocytosis Kit
Dye TypepHrodo™ Red
Emission488 nm
FormatBottle(s)
Product LineBioParticles™, pHrodo™
Quantity100 Assays
Shipping ConditionRoom Temperature
SpeciesE. coli
Unit Size1 kit
A10010Full specifications
5 x 2 mgpHrodo™ Red S. aureus BioParticles™ Conjugate for Phagocytosis
Price: 508.00
Online price: 484.65
Your price:
-
Cell TypeMammalian
ColorRed
DescriptionpHrodo™ Red S. aureus BioParticles™ Conjugate for Phagocytosis
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope
Product TypeConjugate
Dye TypepHrodo™ Red
FormSolid
Product LineBioParticles™, pHrodo™
Quantity5 x 2 mg
Shipping ConditionRoom Temperature
SpeciesS. aureus
Unit Size5 x 2 mg
P35365Full specifications
5 x 1 mgpHrodo™ Green Zymosan BioParticles™ Conjugate for Phagocytosis
Price: 506.00
Online price: 466.65
Your price:
-
ColorGreen
DescriptionpHrodo™ Green Zymosan BioParticles™ Conjugate for Phagocytosis
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Attune Acoustic Focusing Cytometer, Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, High Content Analysis Instrument, Flow Cytometer
Product TypeConjugate
Dye TypepH-Sensitive Dyes
Product LinepHrodo™
Quantity5 x 1 mg
Shipping ConditionRoom Temperature
SpeciesS. cerevisiae
Excitation/Emission509/533
Unit Size5 x 1 mg
Showing 6 of 6
The fluorescence of pHrodo dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. Outside the cell, the dye is non-fluorescent, which eliminates the need for wash steps and quencher dyes. Once internalized, the dye fluoresces brightly within the acidic environments of endosomes and lysosomes, enabling rapid assay development and certainty of results when investigating phagocytic pathways and their regulation by drugs and/or environmental factors. As such, pHrodo dye conjugates can be used in plate readers, fluorescence microscopy imaging, and flow cytometry applications.

The pHrodo Red S. aureus BioParticles Conjugate for Phagocytosis (Cat. No. A10010) offers fast and accurate assay results. pHrodo Red dye conjugates are non-fluorescent outside the cell but fluoresce bright red in phagosomes. The pHrodo Red E. coli BioParticles Conjugate for Phagocytosis (Cat. No. P35361) detects phagocytosis and endocytosis, offers reduced signal variability and improved timing in sensitive experiments, and can be used in multiplex applications with a wide variety of blue, green, and far-red dyes and reporters such as GFP, Fuo-4, calcein, NucBlue, CellEvent Caspase 3/7 green, Mitosox Green, and Mitotracker Deep Red, among many others. The optimal absorption and fluorescence emission maxima of the pHrodo Red BioParticles Conjugate is approximately 560/585 nm, respectively. However, the fluorophore is readily excited with the 488-nm argon-ion laser installed on most flow cytometers.

The pHrodo Red E. coli BioParticles Phagocytosis Kit (Cat. No. A10025) for flow cytometry is designed for rapid and convenient measurement of phagocytic activity in whole blood samples by flow cytometry. The kit includes all the reagents required for assessing particle ingestion and red blood cell lysis.

pHrodo Deep Red dye is a low-background pH sensor dye that shows no signal in neutral conditions and only fluoresces in acidic environments. pHrodo Deep Red dye enables better discrimination of internalized cargo from outside the cell because it has an approximate pKa of 5 and does not fluoresce until it enters the late endosome and lysosome. pHrodo Deep Red dye can be detected using a Cy5 fluorescent filter set and has been validated for use in a variety of applications, including flow cytometry, fluorescent microscopy, high content analysis (HCA), and high throughput screening (HTS). It can also be multiplexed with a wide variety of blue, green, and red dyes and reporters such as GFP and RFPs, Mitosox Green or Red, CellEvent Caspase 3/7 Green or Red, NucBlue, or TMR, among many others.

pHrodo Deep Red E. coli BioParticles Conjugate for Phagocytosis (Cat. No. P35360) is a no-wash, low background, fluorogenic reagent developed for the study of phagocytosis in a live cell system. This conjugate is non-fluorescent outside the cell but fluoresces dark red in phagosomes. The low pKa of the pHrodo Deep Red E. coli BioParticles Conjugate eliminates non-specific signal from non-internalized cargo and only turns on in late endosomes and lysosomes.

The pHrodo Green dye conjugates, like pHrodo Red and pHrodo Deep Red dye conjugates, offer faster and more accurate results than any other phagocytosis assay. pHrodo Green conjugates are non-fluorescent outside the cell at neutral pH but fluoresce bright green at acidic pH, such as that of phagosomes. Use the ready-made pHrodo Green S. aureus BioParticles Conjugate (Cat. No. P35367) and pHrodo Green Zymosan BioParticles Conjugate (Cat. No. P35365) for Phagocytosis in imaging, HCA, HTS, and flow applications.

These pHrodo conjugate products include enough reagent to perform 100 tests when using 100 μL in each well of a 96-well plate, and they can be multiplexed with a wide variety of blue, red, and far-red dye reporters such as Mitosox Red, CellEvent Caspase 3/7 Red, NucBlue, RFPs, and Mitotracker Deep Red, among many others.

pHrodo Red Zymosan BioParticles Conjugate (Cat. No. P35364) is also available, and pHrodo Green dye is available as a conjugate of E. coli BioParticles (Cat. No. P35366).

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Contents & Storage

Store at –20°C, desiccate, and protect from light.

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