Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.
PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ∼30–40 nm to the red and the fluorescence emission maximum is shifted ∼15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.
PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.
Jurkat human T cell leukemia cells. Alexa Fluor 488 annexin V and propidium iodide.
Lightly fixed adult zebrafish intestine stained with the substrate contained in the ELF® 97 Endogenous Phosphatase Detection Kit.
A human pancreatic islet stained with Newport Green™ DCF diacetate and propidium iodide.
Drosophila ovarian egg chamber.
Human keratinocytes. Fluorescein concanavalin A and propidium iodide.
Detection of proliferation in Wil2S Lymphoma B cells
A cryostat section through adult zebrafish kidney that was incubated with the ELF® 97 phosphatase substrate in our ELF® 97 Endogenous Phosphatase Detection Kit and counterstained with propidium iodide.