Invitrogen

RAGE Polyclonal Antibody

Name: RAGE Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-075

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9 Published Figures

18 References

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Datasheet

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Questions & Answers

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Protocols

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Additional Information:

RAGE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 15

RAGE Antibody (PA1-075) in IHC (P)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal... View More

RAGE Antibody in Immunohistochemistry (IHC)

RAGE Antibody in Western Blot, Neutralization (WB, Neu)

Product Image Gallery

RAGE Antibody — Figure 1. High-mobility group box1 (HMGB1) upregulated MUC5AC and MUC5B protein expression, and gene regulation was blocked by a receptor for advanced glycation end products (RAGE) inhibitor. Normal human bronchial epithelial (NHBE) cells were cultured at the air-liquid interface, treated with HMGB1 (10 or 100 ng/mL) for 24 or 48 hours, and analyzed for relative MUC5AC (A) and MUC5B (B) protein levels in the apical media by microtiter plate assay ( see the data supplement). Involvement of the RAGE receptor in HMGB1-induced MUC5AC (C) and MUC5B (D) mRNA expression was tested by pretreatment with a RAGE inhibitor, FPS-ZMI (500 nM), for 1 hour, followed by administration of HMGB1 (10 ng/mL, 24 h). Expression of MUC5AC and MUC5B mRNA was determined by qRT-PCR as described in the data supplement. HMGB1 (10 or 100 ng/mL; 24-48 h) increased AGER (RAGE) mRNA expression (E) and RAGE protein levels as shown by densitometric analysis (F) of Western blots in well-differentiated, primary NHBE cells. A representative Western blot for RAGE is shown in G. RAGE mRNA was determined by qRT-PCR, and RAGE protein levels were compared with vehicle control-treated cells by Western blot, as described in the data supplement. Data are expressed as mean +- SEM. P < 0.05 was considered statistically significant (* P < 0.05, ** P < 0.01, and *** P < 0.001 comparisons between conditions are marked by lines). Two to five independent experiments with six or more replic {TM}

Immunogen image

Immunogen Image RAGE Polyclonal Antibody

Product Details

PA1-075

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1-2 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2269243

Product Specific Information

PA1-075 detects receptor for advanced glycation endproducts (RAGE) from mouse and rat samples.

PA1-075 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post-glycosylation in mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product. Immunohistochemical staining of RAGE in transgenic mouse retina results in staining of the retinal pigmented epithelium and photo receptor cell layers.

The PA1-075 immunogen is a synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE. This sequence is 100% conserved in mouse and 70% conserved in humans. This peptide (Cat. # PEP-194) is available for use in neutralization and control experiments.

Target Information

The Receptor for Advanced Glycation End-products (RAGE) is a gene located on human chromosome 6p21.3, encoding a transmembrane receptor belonging to the immunoglobulin superfamily. RAGE is expressed in various tissues, with significant levels in the lungs, and plays a crucial role in cellular signaling and inflammation. As a receptor, RAGE binds multiple ligands, including advanced glycation end-products (AGEs), amyloid-beta peptide, high mobility group box 1 (HMGB1), and S100/calgranulin proteins, facilitating diverse pathological processes like inflammation, cancer progression, and neurodegeneration. The interaction between RAGE and its ligands triggers intracellular signaling pathways such as NF-kB activation, leading to inflammatory responses and oxidative stress. In the context of chronic diseases like diabetes, Alzheimer's, and cardiovascular diseases, RAGE is a critical mediator, linking metabolic disturbance to cellular dysfunction. Therapeutic targeting of RAGE signaling is under investigation, aiming to mitigate its contribution to inflammatory and degenerative diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: advanced glycation end-products receptor; Advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor variant 2; advanced glycosylation end product-specific receptor variant 3; advanced glycosylation end product-specific receptor variant 4; advanced glycosylation end product-specific receptor variant 5; RAGE-4 ORF3; receptor for advanced glycation end-products variant 20; Receptor for advanced glycosylation end products; sRAGE

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Gene Aliases: Ager; RAGE

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UniProt ID: (Mouse) Q62151

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Entrez Gene ID: (Rat) 81722, (Mouse) 11596

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Function(s)

protein binding identical protein binding S100 protein binding advanced glycation end-product receptor activity high mobility group box 1 binding cell adhesion molecule immunoglobulin superfamily cell adhesion molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAGE Polyclonal Antibody

RAGE Antibody (PA1-075) in IHC (P)

Product Details

PA1-075

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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