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Invitrogen
OCT4 Polyclonal Antibody
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FIGURE: 1 / 12
OCT4 Antibody (PA1-16943) in ICC/IF
Immunocytochemistry analysis of OCT4 in Ntera2 cells. Samples were incubated with OCT4 polyclonal antibody (Product # PA1-16943) using a dilution of 1 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:1,000 dilution for 60 minutes. Cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Product Details
PA1-16943
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate,
Rat
Published species
Bovine,
Cat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is expected to react with swine, feline and bovine samples based on a 93% sequence homology.
Target Information
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; OCT4-B1; OCT4B-variant 2; octamer transcription factor 4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; POU transcription factor; Pou5f1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2; transcriptional regulator
Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OCT4Borf1; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1
UniProt ID: (Human) Q01860, (Mouse) P20263
Entrez Gene ID: (Bovine) 282316, (Human) 5460, (Mouse) 18999, (Rat) 294562
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
ubiquitin protein ligase binding
miRNA binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription coactivator activity
chromatin binding
transcription corepressor activity
transcription factor binding
chromatin DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
protein heterodimerization activity
POU domain binding
HMG box domain binding
regulation of transcription from RNA polymerase II promoter
multicellular organism development
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate specification
blastocyst development
heart induction
endodermal-mesodermal cell signaling
regulation of transcription, DNA-templated
response to wounding
anatomical structure morphogenesis
regulation of asymmetric cell division
regulation of gene expression
BMP signaling pathway
somatic stem cell population maintenance
positive regulation of transcription from RNA polymerase II promoter
cell fate commitment involved in formation of primary germ layer
cardiac cell fate determination
positive regulation of canonical Wnt signaling pathway
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
mesodermal cell fate commitment
endodermal cell fate commitment
ectodermal cell fate commitment
trophectodermal cell differentiation
blastocyst growth
BMP signaling pathway involved in heart induction
transcription, DNA-templated
transcription from RNA polymerase II promoter
response to organic substance
stem cell population maintenance
germ-line stem cell population maintenance
response to retinoic acid
positive regulation of catenin import into nucleus
mRNA transcription from RNA polymerase II promoter
cell fate commitment
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of calcium ion-dependent exocytosis
stem cell differentiation
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
positive regulation of SMAD protein import into nucleus
negative regulation of gene silencing by miRNA
regulation of heart induction by regulation of canonical Wnt signaling pathway
regulation of methylation-dependent chromatin silencing
response to cytokine
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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