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          • Primary Antibodies ›
          • TGF beta-1 Antibodies

          Invitrogen

          TGF beta-1 Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (81) TGF beta-1 antibodies

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          Cite TGF beta-1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (1)
          TGF beta-1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          TGF beta-1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          TGF beta-1 Antibody (PA1-29020) in WB

          Western Blot using TGF beta-1 Polyclonal Antibody (Product # PA1-29020). Mature porcine TGFB1 detected in fibroblasts using TGF beta-1 Polyclonal Antibody (Product # PA1-29020). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Immunohistochemistry (IHC)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          TGF beta-1 Polyclonal Antibody

          Product Details

          PA1-29020

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          -

          Immunohistochemistry (IHC)

          1:200-1:500
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse, Pig, Rat

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein-amino acids 280-391 of mature pro-TGFB1.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.2

          Contains

          0.1% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2202043

          Product Specific Information

          PA1-29020 has been successfully used in immunohistochemistry, Western blot applications.

          Target Information

          TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cytokine; TGF b; TGF b 1; TGF beta; tgf beta 1; TGF beta-1; TGF beta-1 protein; tgf beta1; TGF β; TGF β 1; TGF β1; TGF-B1; TGF-beta 1 protein; TGF-beta precursor; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; Transforming growth factor beta-1; transforming growth factor β1; transforming growth factor, beta 1 (Camurati-Engelmann disease); unnamed protein product

          View more View less

          Gene Aliases: TGF-BETA-1; TGF-beta1; Tgfb; Tgfb-1; TGFbeta1

          View more View less

          UniProt ID: (Pig) P07200, (Mouse) P04202, (Rat) P17246

          View more View less

          Entrez Gene ID: (Pig) 397078, (Mouse) 21803, (Rat) 59086

          View more View less

          Function(s)
          antigen binding type II transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding identical protein binding protein serine/threonine kinase activator activity cytokine activity transforming growth factor beta receptor binding deubiquitinase activator activity macromolecular complex binding growth factor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter MAPK cascade vasculogenesis ureteric bud development epithelial to mesenchymal transition neural tube closure negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of sodium ion transport chondrocyte differentiation hematopoietic progenitor cell differentiation adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains tolerance induction to self antigen aortic valve morphogenesis protein phosphorylation protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process cellular calcium ion homeostasis inflammatory response cell cycle arrest mitotic cell cycle checkpoint epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly Notch signaling pathway salivary gland morphogenesis endoderm development positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration response to wounding positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial to mesenchymal transition positive regulation of fibroblast migration positive regulation of peptidyl-threonine phosphorylation positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production oligodendrocyte development positive regulation of microglia differentiation regulation of striated muscle tissue development cell migration regulation of transforming growth factor beta receptor signaling pathway negative regulation of cell-cell adhesion hyaluronan catabolic process negative regulation of ossification negative regulation of cell growth osteoclast differentiation positive regulation of cell migration positive regulation of bone mineralization positive regulation of histone deacetylation membrane protein intracellular domain proteolysis positive regulation of protein complex assembly lipopolysaccharide-mediated signaling pathway positive regulation of cellular protein metabolic process response to estradiol response to progesterone regulation of interleukin-23 production negative regulation of interleukin-17 production positive regulation of interleukin-17 production receptor catabolic process positive regulation of superoxide anion generation mononuclear cell proliferation positive regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation positive regulation of histone acetylation positive regulation of protein dephosphorylation negative regulation of T cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus positive regulation of odontogenesis myeloid dendritic cell differentiation T cell homeostasis positive regulation of vascular permeability positive regulation of MAP kinase activity protein kinase B signaling positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity ossification involved in bone remodeling regulatory T cell differentiation cell-cell junction organization positive regulation of regulatory T cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of cytolysis negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter muscle cell cellular homeostasis positive regulation of fibroblast proliferation positive regulation of isotype switching to IgA isotypes lymph node development negative regulation of skeletal muscle tissue development negative regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of peptidyl-tyrosine phosphorylation positive regulation of chemotaxis regulation of binding regulation of DNA binding positive regulation of cell division positive regulation of protein kinase B signaling ventricular cardiac muscle tissue morphogenesis face morphogenesis pathway-restricted SMAD protein phosphorylation regulation of SMAD protein import into nucleus positive regulation of SMAD protein import into nucleus mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis regulation of cartilage development negative regulation of biomineral tissue development lens fiber cell differentiation positive regulation of ERK1 and ERK2 cascade response to cholesterol positive regulation of cell cycle arrest cellular response to organic cyclic compound cellular response to transforming growth factor beta stimulus extracellular matrix assembly positive regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of NAD+ ADP-ribosyltransferase activity positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of protein localization to plasma membrane negative regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of receptor clustering transforming growth factor beta receptor signaling pathway involved in heart development embryonic liver development regulation of actin cytoskeleton reorganization positive regulation of transcription regulatory region DNA binding positive regulation of cardiac muscle cell differentiation cell morphogenesis skeletal system development regulation of cell growth response to hypoxia morphogenesis of a branching structure cell activation sprouting angiogenesis columnar/cuboidal epithelial cell maturation CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target heart valve morphogenesis defense response I-kappaB kinase/NF-kappaB signaling negative regulation of neuroblast proliferation heart development skeletal muscle tissue development female pregnancy cell proliferation response to xenobiotic stimulus response to glucose organ morphogenesis response to gamma radiation gene expression regulation of gene expression regulation of metal ion transport neural tube development cell differentiation T cell differentiation mammary gland development organ regeneration positive regulation of exit from mitosis positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation regulation of actin cytoskeleton organization response to vitamin D negative regulation of MyD88-dependent toll-like receptor signaling pathway response to laminar fluid shear stress tube development response to immobilization stress cellular response to platelet-derived growth factor stimulus T cell activation regulation of cell proliferation odontogenesis of dentin-containing tooth myelination positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling surfactant homeostasis proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of MAPK cascade response to ethanol regulation of regulatory T cell differentiation negative regulation of cell differentiation positive regulation of epidermal growth factor receptor signaling pathway lung alveolus development digestive tract development positive regulation of smooth muscle cell proliferation inner ear development positive regulation of epithelial cell proliferation positive regulation of inflammatory response negative regulation of phagocytosis defense response to fungus negative regulation of T cell activation positive regulation of smooth muscle cell differentiation positive regulation of protein metabolic process negative regulation of release of sequestered calcium ion into cytosol neuron apoptotic process regulation of cell cycle phospholipid homeostasis canonical Wnt signaling pathway frontal suture morphogenesis respiratory system development regulation of morphogenesis of a branching structure retina vasculature development in camera-type eye connective tissue development cellular response to mechanical stimulus cellular response to glucose stimulus cellular response to growth factor stimulus cellular response to low-density lipoprotein particle stimulus cellular response to hypoxia cellular response to ionizing radiation cellular response to dexamethasone stimulus positive regulation of mononuclear cell migration odontoblast differentiation T-helper 17 cell lineage commitment positive regulation of branching involved in ureteric bud morphogenesis liver regeneration cellular response to virus positive regulation of protein localization to nucleus positive regulation of extracellular matrix assembly response to salt positive regulation of mesenchymal stem cell proliferation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of vasculature development positive regulation of STAT cascade regulation of epithelial to mesenchymal transition involved in endocardial cushion formation regulation of morphogenesis of an epithelium cellular response to acetaldehyde cellular response to insulin-like growth factor stimulus regulation of organ morphogenesis negative regulation of cell-cell adhesion mediated by cadherin positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of endothelial cell apoptotic process positive regulation of primary miRNA processing apoptotic process lung development regulation of miRNA transcription
          It has to be done as per old AB suggested Products section.

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