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Invitrogen

Estrogen Receptor alpha Polyclonal Antibody

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3 Published Figures
17 References
View all (158) Estrogen Receptor alpha antibodies

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Estrogen Receptor alpha Polyclonal Antibody

Product Details

PA1-308

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
View 8 publications 8 publications

Immunohistochemistry (IHC)

-
View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

1-5 µg/mL
-

Immunocytochemistry (ICC/IF)

1:1,000-1:4,000
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325813

Product Specific Information

PA1-308 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts. Immunohistochemical staining of ER alpha in rat prostate with PA1-308 results in primarily nuclear staining.



The PA1-308 immunogen is a synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha. This peptide sequence is completely conserved between human, rat and mouse and is highly conserved between sheep, porcine, camel, bovine and chicken ER alpha. PA1-308 immunizing peptide (Cat. # PEP-036) is available for use in neutralization and control experiments.



Recombinant ER alpha expressed in baculovirus system (Cat. # RP-310) can be purchased for control experiments in Western blot and gel shift assays.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686N23123; E2 receptor alpha; ER; Er alpha; ER delta E7 isoform; ER-alpha; ER-alpha; del.3*,+1*,4,5,6,7* variant; ER-alpha; del.3*,4,5,6,7*/770 variant; ER-alpha; del.3*,4,5,6,7,8*/862 variant; ER-alpha; del.4*,5*,7 variant; ER-alpha; del.4*,5,6*/548 variant; ER-alpha; del.4*,5,6,7*/714 variant; ER-alpha; del.4*,5,6,7,8*/754 variant; ER-alpha; estrogen receptor alpha delta 3*,4,5,6,7*/819 cingulate gyrus isoform; ER-alpha; estrogen receptor alpha delta 3*,4,5,6,7*/833 superior frontal gyrus isoform; ER-alpha; estrogen receptor alpha delta 4*,5,6,7*/747 substantia nigra isoform; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor-related protein; hER-alpha36; lacks a part of the exon 3, exons 4-6, and part of exon 7; contains an 822 base in frame deletion; lacks most of the exon 3, exons 4-7, and part of exon 8; lacks most of the exon 4, exons 5-6, and part of exon 7; lacks the end of the exon 3, exons 4-7, and part of exon 8; MB1; Nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha; receptor; RP1-130E4.1; steroid hormone receptor; unnamed protein product

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Gene Aliases: ER; ER-alpha; Era; ESR; ESR1; ESRA; Estr; ESTRR; NR3A1; RNESTROR

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UniProt ID: (Human) P03372, (Rat) P06211

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Entrez Gene ID: (Human) 2099, (Rat) 24890

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding TFIIB-class transcription factor binding transcription cofactor binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding steroid binding protein binding calmodulin binding beta-catenin binding zinc ion binding lipid binding TBP-class protein binding enzyme binding protein kinase binding nitric-oxide synthase regulator activity estrogen receptor activity estrogen receptor binding estrogen response element binding identical protein binding sequence-specific DNA binding metal ion binding ATPase binding 14-3-3 protein binding sequence-specific double-stranded DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor binding type 1 metabotropic glutamate receptor binding protein complex binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter antral ovarian follicle growth epithelial cell development chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter signal transduction phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration androgen metabolic process male gonad development negative regulation of gene expression intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to estradiol regulation of toll-like receptor signaling pathway negative regulation of smooth muscle cell apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling steroid hormone mediated signaling pathway negative regulation of sequence-specific DNA binding transcription factor activity response to estrogen positive regulation of nitric oxide biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter fibroblast proliferation positive regulation of fibroblast proliferation stem cell differentiation regulation of inflammatory response positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity RNA polymerase II transcriptional preinitiation complex assembly uterus development vagina development prostate epithelial cord elongation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis regulation of branching involved in prostate gland morphogenesis mammary gland branching involved in pregnancy mammary gland alveolus development epithelial cell proliferation involved in mammary gland duct elongation protein localization to chromatin cellular response to estrogen stimulus cellular response to estradiol stimulus cellular response to oxygen-containing compound negative regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of epithelial cell apoptotic process osteoblast development transcription, DNA-templated positive regulation of phospholipase C activity intracellular receptor signaling pathway regulation of apoptotic process regulation of neuron apoptotic process positive regulation of epidermal growth factor receptor signaling pathway negative regulation of glucose import decidualization positive regulation of retinoic acid receptor signaling pathway negative regulation of smooth muscle cell proliferation Sertoli cell development Sertoli cell proliferation positive regulation of ERK1 and ERK2 cascade negative regulation of triglyceride metabolic process negative regulation of neuron death baculum development
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