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Invitrogen
Connexin 43 Polyclonal Antibody
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FIGURE: 1 / 1
Connexin 43 Antibody (PA1-37497) in IHC (P)
Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human heart tissue sections. To expose target proteins, tissues were boiled for 10 minutes in 10mM citrate buffer, pH 6.0. Following antigen retrieval, tissues were blocked and then probed with a Connexin 43 polyclonal antibody (Product # PA1-37497) at a dilution of 1:100 for at least 10 minutes at room temperature. Tissues were washed ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-37497
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from a segment of the 3 cytoplasmic domain of rat connexin 43
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Gel ice packs
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine and rabbit based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is heart tissue.
Target Information
Connexin 43 (Cx43), encoded by the GJA1 gene, is a widely expressed gap junction protein that plays an important role in direct cell-to-cell communication. Six Cx43 proteins assemble to form a connexon, or hemichannel, which can pair with a connexon on a neighboring cell to create a gap junction channel. These channels allow ions, metabolites, and small signaling molecules to pass between adjacent cells, helping coordinate processes such as electrical conduction, tissue development, wound healing, and cellular homeostasis. Cx43 is especially abundant in tissues such as the heart, brain, bone, and skin, and changes in its expression, localization, or function have been associated with cardiovascular disease, neurological disorders, impaired tissue repair, and cancer. Because of its broad role in intercellular signaling and disease biology, Cx43 is an important target for studying gap junction function and developing potential therapeutic strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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