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Invitrogen
SMAD7 Polyclonal Antibody
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FIGURE: 1 / 9
SMAD7 Antibody (PA1-41506) in IHC (P)
Immunohistochemical analysis of SMAD7 in formalin fixed paraffin-embedded (FFPE) human liver. Samples were incubated in SMAD7 polyclonal antibody (Product # PA1-41506). Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated wit... View More
Product Details
PA1-41506
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Sheep
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Two peptides made from within amino acids 10 and 50 of human SMAD7.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with chimpanzee, canine, pufferfish, xenopus and zebrafish based on sequence homology.
Suggested positive control: A549, HepG2.
Target Information
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ16482; hSMAD7; MAD (mothers against decapentaplegic, Drosophila) homolog 7; MAD homolog 7; MAD homolog 8; madh8; Mothers against decapentaplegic homolog 7; Mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; mothers against DPP homolog 7; mothers against DPP homolog 8; SMAD; SMAD 7; SMAD family member 7; SMAD, mothers against DPP homolog 7; unnamed protein product
Gene Aliases: CRCS3; MADH7; MADH8; SMAD7
UniProt ID: (Human) O15105, (Mouse) O35253
Entrez Gene ID: (Sheep) 101111414, (Human) 4092, (Mouse) 17131
transcription corepressor activity
protein binding
collagen binding
beta-catenin binding
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
metal ion binding
I-SMAD binding
activin receptor binding
transcription regulator inhibitor activity
protein binding, bridging involved in substrate recognition for ubiquitination
DNA binding
transcription factor activity, sequence-specific DNA binding
transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity
transcription regulatory region DNA binding
activin binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
negative regulation of T cell cytokine production
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
anatomical structure morphogenesis
regulation of epithelial to mesenchymal transition
negative regulation of epithelial to mesenchymal transition
negative regulation of transcription by competitive promoter binding
regulation of transforming growth factor beta receptor signaling pathway
cell differentiation
negative regulation of ossification
negative regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
protein complex localization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of activin receptor signaling pathway
protein-containing complex disassembly
adherens junction assembly
response to laminar fluid shear stress
negative regulation of cell differentiation
artery morphogenesis
protein stabilization
ventricular cardiac muscle tissue morphogenesis
regulation of cardiac muscle contraction
regulation of ventricular cardiac muscle cell membrane depolarization
negative regulation of SMAD protein import into nucleus
SMAD protein signal transduction
ventricular septum morphogenesis
cellular response to growth factor stimulus
transforming growth factor beta receptor superfamily signaling pathway
negative regulation of chondrocyte proliferation
positive regulation of chondrocyte hypertrophy
beta-catenin destruction complex disassembly
cellular response to leukemia inhibitory factor
positive regulation of cell-cell adhesion mediated by cadherin
negative regulation of T-helper 17 type immune response
negative regulation of T-helper 17 cell differentiation
transcription, DNA-templated
negative regulation of peptidyl-threonine phosphorylation
positive regulation of cell-cell adhesion
negative regulation of protein ubiquitination
positive regulation of protein ubiquitination
regulation of activin receptor signaling pathway
negative regulation of peptidyl-serine phosphorylation
cellular protein complex localization
intracellular signal transduction
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of ubiquitin-protein transferase activity
pathway-restricted SMAD protein phosphorylation
negative regulation of pathway-restricted SMAD protein phosphorylation
cellular response to transforming growth factor beta stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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