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Invitrogen
Phospho-ALOX5 (Ser523) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-ALOX5 (Ser523) Antibody (PA1-4628) in WB
Western blot of ALOX5 in rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of Phospho-ALOX5 (Ser523) polyclonal antibody (Product # PA1-4628) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1200 units for 30 min).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-4628
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 of human ALOX5 conjugated to KLH
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with non-human primate based on 100% sequence homology.
This antibody is specific for the ~80 kDa 5-LO phosphorylated at Ser523 in Western blots of rat hippocampal lysates.
Target Information
This gene encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5 - Lipoxygenase; 5-lipoxygenase; 5-LO; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase; lipoxygenase; LOX-5; lox5; RP11-67C2.3; unnamed protein product
Gene Aliases: 5-LO; 5-LOX; 5LO; 5LPG; 5LX; F730011J02; LOG5; LOX5A
UniProt ID: (Human) P09917, (Mouse) P48999, (Rat) P12527
Entrez Gene ID: (Human) 240, (Mouse) 11689, (Rat) 25290
arachidonate 5-lipoxygenase activity
arachidonate 12-lipoxygenase activity
iron ion binding
protein binding
oxidoreductase activity
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
arachidonate 8(S)-lipoxygenase activity
metal ion binding
long-chain fatty acid metabolic process
negative regulation of endothelial cell proliferation
leukocyte chemotaxis involved in inflammatory response
leukocyte migration involved in inflammatory response
icosanoid metabolic process
leukotriene metabolic process
response to stress
humoral immune response
negative regulation of angiogenesis
arachidonic acid metabolic process
leukotriene biosynthetic process
lipoxygenase pathway
positive regulation of bone mineralization
lipid oxidation
dendritic cell migration
glucose homeostasis
long-chain fatty acid biosynthetic process
regulation of fat cell differentiation
regulation of inflammatory response
negative regulation of inflammatory response
regulation of insulin secretion
negative regulation of vascular wound healing
negative regulation of wound healing
regulation of inflammatory response to wounding
regulation of cytokine production involved in inflammatory response
regulation of cellular response to oxidative stress
leukotriene A4 biosynthetic process
regulation of reactive oxygen species biosynthetic process
negative regulation of response to endoplasmic reticulum stress
negative regulation of sprouting angiogenesis
positive regulation of leukocyte adhesion to arterial endothelial cell
lipoxin biosynthetic process
leukotriene production involved in inflammatory response
inflammatory response
positive regulation of vasoconstriction
positive regulation of cytochrome-c oxidase activity
response to nutrient
response to hyperoxia
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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