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Invitrogen
CNR2 Polyclonal Antibody
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FIGURE: 1 / 8
CNR2 Antibody (PA1-744) in WB
Western blot analysis of Cannabinoid Receptor II was performed by loading 25 µg of PC-3 (lane 1) and Jurkat (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Cannabinoid Receptor II polyclonal antibody (Product # PA1-744) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Pl... View More
Product Details
PA1-744
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Dog,
Hamster,
Horse,
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A fusion protein containing the first 33 amino acid residues from human CB2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-744 detects cannabinoid receptor 2 (CB2) from human tissues.
PA1-744 has been successfully used in Western blot, FACS, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa band from human spinal cord homogenate, and a ~40 kda band from RBL2H3 cells, representing the different glycosylated forms of CB2.
PA1-744 antigen is a fusion protein containing the first 33 amino acid residues from human CB2.
Target Information
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cannabinoid Receptor 2; cannabinoid receptor 2 (macrophage); CB-2; CB2R; CNR3; CX5; HCB2; HGNC:2160; OTTHUMP00000044841; RP11-4M23.1
Gene Aliases: CB-2; CB2; CB2A; CB2B; CNR2; CX5
UniProt ID: (Human) P34972
Entrez Gene ID: (Human) 1269
response to amphetamine
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
adenylate cyclase-activating G-protein coupled receptor signaling pathway
leukocyte chemotaxis
negative regulation of synaptic transmission, GABAergic
response to lipopolysaccharide
negative regulation of mast cell activation
cannabinoid signaling pathway
negative regulation of action potential
trans-synaptic signaling by endocannabinoid, modulating synaptic transmission
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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