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Invitrogen
RAB3A Polyclonal Antibody
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FIGURE: 1 / 7
RAB3A Antibody (PA1-770) in ICC/IF
Immunofluorescence analysis of RAB3A was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB3A Rabbit Polyclonal Antibody (Product # PA1-770) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at... View More
Product Details
PA1-770
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Hamster,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) A S A T D S R Y G Q K E S S D Q N(18) C of human RAB3A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-770 detects rab 3A from canine, hamster, human, mouse and rat tissues and cells.
PA1-770 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~21 kDa protein representing rab 3A from Hepa1 cell lysate.
PA1-770 immunogen is a synthetic peptide corresponding to residues M(1) A S A T D S R Y G Q K E S S D Q N(18) C of human RAB3A. This sequence is completely conserved between human, mouse, and rat rab 3A. PA1-770 immunizing peptide (Cat. # PEP-098) is available for use in neutralization and control experiments.
Recombinant human rab 3A (Cat # RP-770) protein can be purchased for control experiments in Western blot applications.
Target Information
Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D. These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters. Specifically, Rab 3s are thought to play a key role in presynaptic vesicle trafficking and priming.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C18A3.6; RAB3A, member RAS oncogene family; RAS-associated protein RAB3A; Ras-related protein Rab-3A; ras-related protein Rab-3A-like protein; Ras-related small GTP binding protein 3A; unnamed protein product
Gene Aliases: RAB3; RAB3A
UniProt ID: (Human) P20336, (Rat) P63012, (Mouse) P63011
Entrez Gene ID: (Human) 5864, (Dog) 484826, (Chinese hamster) 100759024, (Rat) 25531, (Mouse) 19339
respiratory system process
intracellular protein transport
vesicle docking involved in exocytosis
mitochondrion organization
neuromuscular synaptic transmission
axonogenesis
metabolic process
protein secretion
post-embryonic development
synaptic vesicle exocytosis
synaptic vesicle maturation
regulation of exocytosis
lung development
regulation of synaptic vesicle fusion to presynaptic membrane
Rab protein signal transduction
positive regulation of ATPase activity
synaptic vesicle recycling
regulation of short-term neuronal synaptic plasticity
maintenance of presynaptic active zone structure
sensory perception of touch
response to electrical stimulus
protein localization to plasma membrane
positive regulation of regulated secretory pathway
plasma membrane repair
exocytosis
protein transport
calcium ion regulated exocytosis
insulin secretion
lysosome localization
constitutive secretory pathway
regulated exocytosis
positive regulation of exocytosis
synaptic vesicle transport
establishment of localization in cell
acrosomal vesicle exocytosis
evoked neurotransmitter secretion
synaptic vesicle clustering
regulation of presynaptic dense core granule exocytosis
regulation of plasma membrane repair
transport
small GTPase mediated signal transduction
regulation of long-term synaptic potentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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