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Invitrogen
Goat anti-Rat IgG2b Secondary Antibody, HRP
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FIGURE: 1 / 2
Rat IgG2b Secondary Antibody (PA1-84710) in WB
Western blot was performed using Goat anti-Rat IgG2b Secondary Antibody, HRP (Product # PA1-84710). Membrane enriched extracts of HeLa (Lane 1), Hep G2 (Lane 2, 3, 4) and LnCaP (Lane 5, 6, 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were transferred onto a nitrocellulose membrane (Product # IB33002) by iBlot™ 3 Western Blot Transfer Device (Product # IB31001). The blot was probed with IkB alpha Recombinant Rat Monoclonal Antibody (MFRDTRK) (Product # 740061T, 1:500) and HSP70 Po... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-84710
Applications
Tested Dilution
Western Blot (WB)
1:1,000-1:30,000
Immunohistochemistry (Paraffin) (IHC (P))
1:200-1:500
Immunohistochemistry (Frozen) (IHC (F))
1:200-1:500
ELISA (ELISA)
1:10,000-1:100,000
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Proprietary Immunogen.
Conjugate
HRP
HRP
HRP
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Affinity chromatography
Storage buffer
PBS with 0.2% BSA
Contains
0.05% ProClin 300
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG2b
Antibody Form
Whole Antibody
Product Specific Information
Goat anti Rat IgG2b antibody recognizes rat IgG2b as assessed by immunoelectrophoresis and ELISA.Goat anti Rat IgG2b antibody may cross react with IgG2b from other species.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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