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Invitrogen
HDAC1 Polyclonal Antibody, Alexa Fluor™ 647
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FIGURE: 1 / 1
HDAC1 Antibody (PA1-860-A647) in ICC/IF
Immunofluorescent analysis of HDAC1 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HDAC1 Polyclonal Antibody, AlexaFluor 647 conjugate (Product # PA1-860-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Imag... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-860-A647
Product Specifications
Species Reactivity
Dog,
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1
Conjugate
Alexa Fluor™ 647
Alexa Fluor™ 647
Alexa Fluor™ 647
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1860A647 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.
The PA1860A647 immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. This immunizing peptide (Cat. # PEP-069) is available for use in neutralization and control experiments.
Target Information
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686H12203; HD1; HDAC1; histone deacetylase 1; histone deacetylase 1-like protein; protein deacetylase HDAC1; protein deacylase HDAC1; protein decrotonylase HDAC1; reduced potassium dependency, yeast homolog-like 1; RP4-811H24.2; unnamed protein product
Gene Aliases: GON-10; HD1; Hdac1-ps; KDAC1; MommeD5; RPD3; RPD3L1
UniProt ID: (Human) Q13547, (Mouse) O09106, (Rat) Q4QQW4
Entrez Gene ID: (Human) 3065, (Dog) 487309, (Mouse) 433759, (Rat) 297893, (Chinese hamster) 100752721
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcription corepressor binding
p53 binding
DNA binding
chromatin binding
transcription corepressor activity
histone deacetylase activity
protein binding
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
enzyme binding
nucleosomal DNA binding
protein deacetylase activity
Krueppel-associated box domain binding
histone deacetylase binding
NF-kappaB binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
E-box binding
DNA-binding transcription factor binding
histone deacetylase activity, hydrolytic mechanism
protein decrotonylase activity
histone decrotonylase activity
protein lysine delactylase activity
promoter-specific chromatin binding
deacetylase activity
macromolecular complex binding
negative regulation of transcription from RNA polymerase II promoter
response to amphetamine
chromatin organization
chromatin remodeling
methylation-dependent chromatin silencing
regulation of transcription from RNA polymerase II promoter
endoderm development
circadian rhythm
positive regulation of cell proliferation
negative regulation of cell proliferation
response to xenobiotic stimulus
epidermal cell differentiation
positive regulation of gene expression
negative regulation of gene expression
hippocampus development
negative regulation of Wnt signaling pathway
neuron differentiation
negative regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
response to caffeine
heterochromatin assembly
response to lipopolysaccharide
positive regulation of interleukin-1 production
positive regulation of tumor necrosis factor production
circadian regulation of gene expression
positive regulation of intracellular estrogen receptor signaling pathway
cellular response to oxidative stress
cellular response to platelet-derived growth factor stimulus
odontogenesis of dentin-containing tooth
regulation of cell fate specification
embryonic digit morphogenesis
negative regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of neuron apoptotic process
negative regulation by host of viral transcription
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
positive regulation of smooth muscle cell proliferation
positive regulation of oligodendrocyte differentiation
response to hyperoxia
negative regulation of androgen receptor signaling pathway
hair follicle placode formation
eyelid development in camera-type eye
fungiform papilla formation
cellular response to tumor necrosis factor
negative regulation of canonical Wnt signaling pathway
negative regulation of stem cell population maintenance
positive regulation of stem cell population maintenance
positive regulation of chemokine (C-X-C motif) ligand 2 production
positive regulation of type B pancreatic cell apoptotic process
regulation of stem cell differentiation
negative regulation of intrinsic apoptotic signaling pathway
negative regulation of myotube differentiation
regulation of embryonic development
negative regulation of cell communication
negative regulation of signaling
positive regulation of protein modification process
negative regulation of peptidyl-lysine acetylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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