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Invitrogen
Phosphothreonine Polyclonal Antibody
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Product Details
PA1-86893
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phosphothreonine conjugated to KLH
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Wet ice
RRID
Product Specific Information
For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM NaF should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Target Information
Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine. There are three amino acids that are typically phosphorylated in eukaryotes: serine, threonine, and tyrosine. Threonine residues in endogenous proteins undergo phosphorylation through the action of a threonine kinase. Small amounts of free phosphothreonine can be detected in urine Antibodies against phosphothreonine can be used as an identifier in the analysis of peptides, proteins, and enzymes. [National Center for Biotechnology Information.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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