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Invitrogen
DNMT3B Polyclonal Antibody
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FIGURE: 1 / 12
DNMT3B Antibody (PA1-884) in IHC (P)
Immunohistochemistry analysis of DNA Methyltransferase 3 showing positive staining in the nucleus and cytoplasm of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blo... View More
Product Details
PA1-884
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-884 detects DNA methyltransferase 3 from human and mouse tissues and cells as well as recombinant human Dnmt3. This antibody has been used to detect both recombinant and endogenous DNMT3b and recombinant DNMT3a.
PA1-884 has been successfully used in Immunohistochemistry (paraffin) and Western blot procedures. By Western blot, this antibody detects DNMT3 from P19 nuclear extracts.
The PA1-884 immunogen is a synthetic peptide corresponding to residues 1-14 of mouse DNMT3b. This sequence is 79% conserved between human and mouse. PA1-884 immunizing peptide (Cat. # PEP-117) is available for use in neutralization and control experiments.
Target Information
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: de novo DNA methyltransferase; DNA (cytosine-5)-methyltransferase 3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA cytosine-5--methyltransferase 3 beta; DNA methyltransferase HsaIIIB; DNA methyltransferase MmuIIIB; DNA MTase HsaIIIB; DNA MTase MmuIIIB; Dnmt3; Dnmt3b; DNMT3B1; DNMT3B4; DNMT3B5; DNMT3B7; m.MmuIIIB; MGC1090; unnamed protein product
Gene Aliases: DNMT3B; FSHD4; ICF; ICF1; M.HsaIIIB; MmuIIIB
UniProt ID: (Human) Q9UBC3, (Mouse) O88509
Entrez Gene ID: (Human) 1789, (Mouse) 13436
DNA binding
transcription corepressor activity
DNA (cytosine-5-)-methyltransferase activity
protein binding
methyltransferase activity
zinc ion binding
DNA-methyltransferase activity
transferase activity
metal ion binding
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
chromatin binding
histone deacetylase binding
unmethylated CpG binding
DNA methyltransferase
negative regulation of transcription from RNA polymerase II promoter
regulation of gene expression
positive regulation of gene expression
methylation
DNA methylation
methylation-dependent chromatin silencing
regulation of gene expression by genetic imprinting
DNA methylation on cytosine within a CG sequence
protein complex localization
positive regulation of neuron differentiation
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
positive regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
inactivation of X chromosome by DNA methylation
cellular response to amino acid stimulus
C-5 methylation of cytosine
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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