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Invitrogen
PMCA1 ATPase Polyclonal Antibody
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FIGURE: 1 / 12
PMCA1 ATPase Antibody (PA1-914) in IHC (P)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyc... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-914
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig,
Rat
Published species
Human,
Mouse,
Non-human primate,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the residues A(5) N N S V A Y S G V K N S I K E A N(22) of rat PMCA1 ATPase.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-914 detects calcium pump of the plasma membrane 1a (PMCA1a ATPase) and 1b from rat, human and porcine tissues.
PA1-914 has been successfully used in Western blot, immunohistochemistry (paraffin and frozen), and ELISA procedures. By Western blot, this antibody detects ~134 kDa and ~130 kDa proteins representing PMCA1a and 1b ATPase from rat, human and porcine samples.
The PA1-914 immunogen is a synthetic peptide corresponding to the residues A(5) N N S V A Y S G V K N S I K E A N(22) of rat PMCA1 ATPase.
Target Information
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATPase, Ca++ transporting, plasma membrane 1; calcium pump; plasma membrane Ca2+ pump (PMCA1b); plasma membrane calcium ATPase; plasma membrane calcium pump; plasma membrane calcium-ATPase isoform 1 alternative splice variant k at splice site B and splice variant b at splice site C; PMCA1; PMCA1kb
Gene Aliases: MRD66; PMCA1; Pmca1a; Pmca1b; Pmca1c; PMCA1kb
UniProt ID: (Human) P20020, (Pig) P23220, (Rat) P11505
Entrez Gene ID: (Human) 490, (Pig) 397636, (Rat) 29598
negative regulation of cytokine production
regulation of vascular smooth muscle contraction
cellular calcium ion homeostasis
regulation of blood pressure
positive regulation of bone mineralization
ion transmembrane transport
regulation of cytosolic calcium ion concentration
negative regulation of cytosolic calcium ion concentration
positive regulation of calcium ion transport
calcium ion transmembrane transport
regulation of cellular response to insulin stimulus
regulation of cardiac conduction
calcium ion export from cell
neural retina development
calcium ion transport
cellular response to vitamin D
cellular response to corticosterone stimulus
calcium ion export
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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