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Invitrogen
PMCA3 ATPase Polyclonal Antibody
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FIGURE: 1 / 7
PMCA3 ATPase Antibody (PA1-916) in IHC (P)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyc... View More
Product Details
PA1-916
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig,
Rat
Published species
Human,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: A(5) N S S I E F H P K P Q Q Q R E V P(22)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-916 detects calcium pump of the plasma membrane 3 (PMCA3 ATPase) from rat, human and porcine tissues.
PA1-916 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~127 kDa protein representing PMCA3a and PMCA3b ATPase from rat brain microsomal fractions.
PA1-916 immunizing peptide corresponds to amino acid residues 5-22 from rat PMCA3 ATPase protein. This sequence is completely conserved between rat and human PMCA3 ATPase.
Target Information
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATPase, Ca++ transporting, plasma membrane 3; cilia and flagella associated protein 39; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 3; plasma membrane calcium pump; Plasma membrane calcium pump isoform 3; plasma membrane calcium-ATPase isoform 3, splice variant a at alternative splice site C; Plasma membrane calcium-transporting ATPase 3; PMCA3; unnamed protein product
Gene Aliases: ATP2B3; CFAP39; CLA2; OPCA; PMCA3; PMCA3a; SCAX1
UniProt ID: (Human) Q16720, (Rat) Q64568
Entrez Gene ID: (Human) 492, (Pig) 100520061, (Rat) 29599
nucleotide binding
calcium-transporting ATPase activity
protein binding
calmodulin binding
ATP binding
calcium ion transmembrane transporter activity
ATPase activity
metal ion binding
P-type calcium transporter activity involved in regulation of presynaptic cytosolic calcium ion concentration
PDZ domain binding
primary active transporter
ion transport
calcium ion transport
ion transmembrane transport
regulation of cytosolic calcium ion concentration
calcium ion transmembrane transport
regulation of presynaptic cytosolic calcium ion concentration
regulation of cardiac conduction
calcium ion export from cell
neural retina development
brain development
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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