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PSMD1 Polyclonal Antibody
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FIGURE: 1 / 3
PSMD1 Antibody (PA1-973) in WB
Western blot analysis was performed on whole cell extracts (30 µglysate) of MCF7 (Lane 1), PC-3 (Lane 2), Hep G2 (Lane 3), SK-OV-3 (Lane 4), HeLa (Lane 5), COS-7 (Lane 6), Jurkat (Lane 7), A-431 (Lane 8) and tissue extracts (30 µglysate) of Mouse Testis (Lane 9) and Rat Testis (Lane 10). The blot was probed with Anti-PSMD1 Rabbit Polyclonal Antibody (Product # PA1-973, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilut... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-973
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: A(929) A H G P K I E E E E Q E P E P P E P F E Y I D D(953)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-973 detects proteasome 19S subunit S1 from from human cells.
PA1-973 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract.
PA1-973 immunizing peptide corresponds to amino acid residues 929-953 from human proteasome 19S subunit S1 protein.
Target Information
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 26S proteasome p112 subunit; 26S proteasome regulatory subunit RPN2; 26S proteasome regulatory subunit S1; 26S proteasome subunit p112; 26S proteasome, subunit p112; MGC133040; MGC133041; proteasome (prosome, macropain) 26S subunit, non-ATPase, 1; proteasome 26S non-ATPase subunit 1; unnamed protein product
Gene Aliases: 2410026J11Rik; P112; Rpn2; S1
UniProt ID: (Human) Q99460, (Rat) O88761, (Mouse) Q3TXS7
Entrez Gene ID: (Human) 5707, (Bovine) 511803, (Rat) 83806, (Mouse) 70247
response to oxidative stress
proteasomal protein catabolic process
regulation of protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
cellular response to type I interferon
regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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