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Invitrogen
HSP90 alpha Polyclonal Antibody
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FIGURE: 1 / 46
HSP90 alpha Antibody (PA3-013) in ICC/IF
Immunofluorescent analysis of Heat Shock Protein 86 (HSP86, green) in HeLa cells and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HSP86 polyclonal antibody (Product # PA3-013), at a dilution of 1:100 for at least 1 hour at ro... View More
Product Details
PA3-013
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Sheep,
Rat
Published species
Human,
Mouse,
Non-human primate,
Rabbit
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-013 detects heat shock protein 90 (HSP90) from human, monkey, mouse, sheep (adrenal) and rat tissues and cells. This antibody does not detect HSP84.
PA3-013 has been successfully used in Western blot, immunofluorescence, immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects an ~86 kDa protein representing HSP86 from HeLa cell lysate. Immunofluorescence staining of HSP86 in HeLa cells with PA3-013 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP86 exists primarily as homodimers in HeLa cells. Furthermore, the antibody is capable of precipitating HSP86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.
The PA3-013 immunogen is a synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.
Target Information
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein alpha; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen; unnamed protein product
Gene Aliases: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; Hsp103; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2
UniProt ID: (Human) P07900, (Mouse) P07901, (Rat) P82995
Entrez Gene ID: (Sheep) 100127209, (Human) 3320, (Mouse) 15519, (Rat) 299331
nucleotide binding
UTP binding
CTP binding
RNA binding
mRNA binding
protein binding
ATP binding
GTP binding
hydrolase activity
ATPase activity
sulfonylurea receptor binding
protein phosphatase binding
MHC class II protein complex binding
nitric-oxide synthase regulator activity
TPR domain binding
ubiquitin protein ligase binding
dATP binding
identical protein binding
protein homodimerization activity
histone deacetylase binding
protein binding involved in protein folding
ion channel binding
tau protein binding
GTPase binding
Rho GDP-dissociation inhibitor binding
unfolded protein binding
DNA polymerase binding
scaffold protein binding
disordered domain specific binding
ATP-dependent protein folding chaperone
enzyme-substrate adaptor activity
protein tyrosine kinase binding
protein kinase binding
poly(A) RNA binding
Hsp90 family chaperone
neuron migration
activation of innate immune response
positive regulation of defense response to virus by host
skeletal muscle contraction
protein folding
mitochondrial transport
response to unfolded protein
telomere maintenance via telomerase
response to heat
response to cold
response to xenobiotic stimulus
response to salt stress
positive regulation of lamellipodium assembly
cardiac muscle cell apoptotic process
protein import into mitochondrial matrix
regulation of protein ubiquitination
positive regulation of telomere maintenance via telomerase
positive regulation of protein polymerization
positive regulation of interferon-beta production
regulation of protein localization
cellular response to heat
protein refolding
response to cocaine
positive regulation of protein import into nucleus
regulation of apoptotic process
regulation of protein complex assembly
protein unfolding
response to estrogen
positive regulation of nitric oxide biosynthetic process
positive regulation of protein catabolic process
positive regulation of cell size
nitric oxide metabolic process
response to antibiotic
protein stabilization
chaperone-mediated protein complex assembly
positive regulation of cardiac muscle contraction
chaperone-mediated autophagy
cellular response to virus
regulation of postsynaptic membrane neurotransmitter receptor levels
neurofibrillary tangle assembly
telomerase holoenzyme complex assembly
nitric oxide biosynthetic process
response to stress
positive regulation of protein import into nucleus, translocation
protein import into mitochondrial outer membrane
positive regulation of cytotoxic T cell differentiation
regulation of catalytic activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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