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Invitrogen
TRIM25 Polyclonal Antibody
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Product Details
PA5-110701
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein corresonding to Human TRIM25. Recombinant protein control fragment (Product #RP-89334).
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: FCPEHSECIC HICLVEHKTC SPASLSQASA DLEATLRHKL TVMYSQINGA SRALDDVRNR QQDVRMTANR KVEQLQQEYT EMKALLDASE TTSTRKIKEE EKRVNSKFDT IYQILLKKKS EIQTL
Highest antigen sequence indentity to the following orthologs: Mouse - 72%, Rat - 71%.
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase; RING-type E3 ubiquitin transferase TRIM25; tripartite motif protein TRIM25; tripartite motif-containing 25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147; zinc finger protein 147 (estrogen-responsive finger protein); zinc finger protein-147
Gene Aliases: EFP; RNF147; TRIM25; Z147; ZNF147
UniProt ID: (Human) Q14258
Entrez Gene ID: (Human) 7706
protein monoubiquitination
translesion synthesis
negative regulation of type I interferon production
ERAD pathway
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to estrogen
innate immune response
regulation of viral entry into host cell
negative regulation of viral entry into host cell
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
defense response to virus
interferon-gamma-mediated signaling pathway
cell-cell adhesion
regulation of viral release from host cell
negative regulation of viral release from host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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