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Invitrogen
BMI-1 Polyclonal Antibody
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FIGURE: 1 / 12
BMI-1 Antibody (PA5-110999) in ICC/IF
Immunofluorescence analysis of polycomb group protein Bmi1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BMI-1 Polyclonal Antibody (Product # PA5-110999) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus ... View More
Product Details
PA5-110999
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein corresonding to Human BMI-1. Recombinant protein control fragment (Product #RP-95137).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: RPTCKRMKIS HQRDGLTNAG ELESDSGSDK ANSPAGGIPS TSSCLPSPST PVQSPHPQFP HISSTMNGTS NSPSGNHQSS FANRPRKSSV NGSSATSSG
Highest antigen sequence indentity to the following orthologs: Mouse - 95%, Rat - 95%.
Target Information
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B lymphoma Mo-MLV insertion region 1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; COMMD3; murine leukemia viral (bmi-1) oncogene homolog; Pcgf4 antibody; Polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; Polycomb group RING finger protein 4; putative; RING finger protein 51; RP11-573G6.1
Gene Aliases: AW546694; Bmi-1; BMI1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
UniProt ID: (Human) P35226, (Mouse) P25916
Entrez Gene ID: (Human) 648, (Rat) 307151, (Mouse) 12151
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
in utero embryonic development
DNA methylation
humoral immune response
brain development
regulation of gene expression
histone acetylation
histone ubiquitination
rostrocaudal neural tube patterning
hemopoiesis
positive regulation of B cell proliferation
positive regulation of immature T cell proliferation in thymus
somatic stem cell division
positive regulation of fibroblast proliferation
embryonic skeletal system morphogenesis
embryonic skeletal system development
regulation of catalytic activity
positive regulation of ubiquitin-protein transferase activity
cellular response to interleukin-1
cellular response to dexamethasone stimulus
negative regulation of apoptotic signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular process
chromatin modification
hematopoietic stem cell homeostasis
chromatin organization
chromatin remodeling
methylation-dependent chromatin silencing
segment specification
negative regulation of gene expression, epigenetic
regulation of kidney development
apoptotic signaling pathway
regulation of adaxial/abaxial pattern formation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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