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Invitrogen
DTL Polyclonal Antibody
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Product Details
PA5-111035
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein corresonding to Human DTL. Recombinant protein control fragment (Product #RP-105875).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Amount
5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: RVKRRLDSSC LESVKQKCVK SCNCVTELDG QVENLHLDLC CLAGNQEDLS KDSLGPTKSS KIEGAGTSIS EPPSPISPYA SESCGTLPLP LRPCGEGSE
Highest antigen sequence indentity to the following orthologs: Rat - 87%, Mouse - 88%.
Target Information
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: contains WD-40 repeats; DDB1- and CUL4-associated factor 2; Denticleless protein homolog; L2DTL; CDT2; Lethal(2) denticleless protein homolog; RA-regulated nuclear matrix-associated protein; RAMP; Retinoic acid-regulated nuclear matrix-associated protein; unnamed protein product
Gene Aliases: CDT2; CDW1; DCAF2; DTL; L2DTL; RAMP
UniProt ID: (Human) Q9NZJ0
Entrez Gene ID: (Human) 51514
protein polyubiquitination
DNA replication
ubiquitin-dependent protein catabolic process
protein monoubiquitination
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
response to UV
positive regulation of catabolic process
positive regulation of G2/M transition of mitotic cell cycle
protein ubiquitination
translesion synthesis
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of protein catabolic process
rhythmic process
positive regulation of protein metabolic process
regulation of cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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