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Invitrogen
SMAD5 Polyclonal Antibody
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Product Details
PA5-111474
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein corresonding to Human SMAD5. Recombinant protein control fragment (Product #RP-100022).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Amount
5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PDDQMGQDNS QPMDTSNNMI PQIMPSISSR DVQPVAYE
Highest antigen sequence indentity to the following orthologs: Rat - 100%, Mouse - 89%.
Target Information
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chromosome 5 Mad homolog; DKFZp781C1895; DKFZp781O1323; hSmad5; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; MADH5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5; unnamed protein product
Gene Aliases: DWFC; JV5-1; MADH5; SMAD5
UniProt ID: (Human) Q99717
Entrez Gene ID: (Human) 4090
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
DEAD/H-box RNA helicase binding
ubiquitin protein ligase binding
metal ion binding
I-SMAD binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
ossification
osteoblast differentiation
ureteric bud development
Mullerian duct regression
osteoblast fate commitment
cardiac conduction system development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular iron ion homeostasis
signal transduction
transforming growth factor beta receptor signaling pathway
germ cell development
anatomical structure morphogenesis
embryonic pattern specification
negative regulation of gene expression
cell differentiation
erythrocyte differentiation
BMP signaling pathway
developmental process
negative regulation of apoptotic process
positive regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
cartilage development
cardiac muscle contraction
bone development
SMAD protein signal transduction
cellular response to growth factor stimulus
transforming growth factor beta receptor superfamily signaling pathway
negative regulation of Fas signaling pathway
anti-Mullerian hormone signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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